Fig. 3

Plasma galectin-9 outperforms viral load in predicting future disease progression. Histogram of post-acute time point (DPI ≥ 50) for SIV-infected animals. Samples were categorized based on MTO level (a) or nearing euthanasia (b). a Among 9 biomarkers tested, the two best markers for classifying samples in higher or lower MTO groups, i.e. MTO higher or lower than 13.2% as estimated previously [2], were plasma galectin-9 and plasma viral load. b Among 10 biomarkers, the two best markers to classify samples based on time to medical cull were MTO and plasma galectin-9. ROC curves were calculated, and the optimal threshold was determined with Youden's J statistic to maximize the sum of sensitivity and specificity. In case of a tie, thresholds with the best specificity were selected. The dashed lines represent this threshold. Thr. = threshold value for the measured parameter, Sp. and Se. = specificity and sensitivity at the threshold, respectively. If time to medical cull could not be determined due to the end of the study censorship, samples were not included in panel b