Fig. 3

Confirmation of pNAPE-PLD colonization of the mice lungs. At the end of the experimental protocol the BALF from pNAPE-LP/Palmitate group displays significant levels of erythromycin resistant CFU compared to the Vehicle group (A). Once picked and amplified, these cells are revealed to express the NAPE-PLD enzyme as confirmed by immunofluorescence (C), and western blot analysis (46 kDa) (housekeeping protein GroEL, 57 kDa) (D, E). The HPLC-MS analysis of homogenates of lungs revealed a significant increase in the levels of PEA only in pNAPE-LP/Palmitate group (B). Results are expressed as mean ± SD of n = 7 or 9 experiments performed in triplicate. *** p < 0.001, **p < 0.01, * p < 0.05 vs. Vehicle, n.d.=non detectable