Fig. 5

Prophylactic administration of pNAPE-LP/Palmitate reduces pro-inflammatory markers expression and body temperature in mice. Western blot analysis on lung specimens to detect TLR4 (95 kDa), cleaved Caspase 1 (50 kDa), NLRP3 (120 kDa) and the housekeeping protein β-actin (40 kDa) (A), and the relative densitometric quantification (B, C, D) showing the protective effect of pNAPE-LP/palmitate treatment on SP-induced lung injury. pNAPE-LP + palmitate treatment reduces ILs (E, F), TNF-alpha (G) and CRP (H), thus reducing the body temperature (I) in SP-challenged mice. Results are expressed as mean ± SEM of n = 5 experiments performed in triplicate. *** p < 0.001, **p < 0.01, * p < 0.05 vs. SP [500 µg/Kg]