Fig. 5

Cholesterol depletion inhibits MDA-MB 231 cell migration. Cells were cultured up to 90 %-100 % confluence and then scratched wound lines were created by a micropipette tip (0 h). Some of the scratched cultures were treated with MbCD, Bio, Wnt3a, Wnt5a, Dkk-1, or combinations with these molecules. After 2 h of treatment the culture media were changed to MbCD-free fresh media and cells were analyzed by phase-contrast microscopy after 8 or 24 h of treatment. Note that untreated cells (control) and cells treated with Dkk-1 completely covered the scratched areas of the dish after 24 h of culture while in MbCD- and Bio-treated cultures it is possible to see a relatively large empty area in the culture dish after 24 h of treatment