Fig. 6

Cell migration is inhibited in a similar extent with MbCD and Bio, but not with Dkk-1. Cells were cultured up to 90 %-100 % confluence and then scratched wound lines were created by a micropipette tip (0 h). Some of the scratched cultures were treated with MbCD, Bio, Wnt3a, Wnt5a, Dkk-1, or combinations with these molecules. After 2 h of treatment with MbCD the culture media was changed to MbCD-free fresh media. Wound areas were quantified after 2, 12 and 24 h in each condition. Note that 60 % of the scratched area was still empty after 24 h of MbCD treatment, while cells treated with Dkk-1 completely covered the scratched areas at the same time point. Each point represents the mean of 3 independent experiments