Fig. 4
From: Optimization of CFTR-mRNA transfection in human nasal epithelial cells
Statistical evaluation of the wtCFTR-mRNA dose dependency. a Shown are the short-circuit values (Isc) of CFTR activation by cAMP addition and the inhibition by CFTRinh172 application in HNE cells. The stepwise decrease of the transfection dose (2.4 μg/cm2, 1.2 μg/cm2, 0.6 μg/cm2 and 0.3 μg/cm2) produced CFTR activation after the cAMP application. The most efficient CFTR activation was reached using the mRNA dose of 0.6 μg/cm2 (9.3 ± 2.0 μA/ cm2; n = 19) compared to the controls cells (2.8 ± 0.5 μA/ cm2; n = 39). The subsequent reduction to a concentration of 0.3 μg/cm2 did not show any proper activation of CFTR. b The inhibition by the CFTRinh172 is also increased in the wtCFTR-mRNA transfected cells. Although, we could not observe a reduction by the inhibitor in the control cells, we found a 72.93 % inhibition of the cAMP activated CFTR current in the wtCFTR-mRNA transfected cells with a dose of 0.6 μg/cm2 (n = 19). The higher rate of inhibition in transfected HNE cells with the dose of 0.6 μg/cm2 may demonstrate that more CFTR molecules are expressed after mRNA transfection than in control cells (non-transfected) (*p < 0.05; **p < 0.01)