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Table 5 Precision of DLX1, HOXC6, and KLK3 mRNA-based assays

From: Analytical validation of an mRNA-based urine test to predict the presence of high-grade prostate cancer

   DLX1 HOXC6 KLK3
  Copies/RT-PCR 400 100 76 800 200 125 400 100 67
Overall precision Avg Cp 31.7 33.8 34.3 31.2 33.4 34.1 31.3 33.3 33.8
SD Cp 0.2 0.2 0.3 0.1 0.2 0.3 0.1 0.2 0.3
Intra-run precision (repeatability) Avg Cp 31.6 33.8 34.2 31.1 33.4 34.1 31.3 33.2 33.4
SD Cp 0.1 0.3 0.3 0.1 0.2 0.2 0.1 0.3 0.5
Inter-run precision (reproducibility) Avg Cp 31.8 33.8 34.4 31.2 33.4 34.1 31.4 33.4 33.9
SD Cp 0.1 0.3 0.4 0.1 0.2 0.2 0.2 0.2 0.4
Inter-instrument precision Avg Cp 31.7 33.9 34.3 31.2 33.4 34.2 31.4 33.4 33.9
SD Cp 0.1 0.2 0.4 0.1 0.3 0.2 0.1 0.2 0.3
Inter-operator precision Avg Cp 31.7 33.8 34.5 31.1 33.4 33.9 31.4 33.4 33.9
SD Cp 0.1 0.2 0.3 0.3 0.2 0.2 0.1 0.2 0.3
  1. Avg average, Cp crossing point, DLX1 Distal-less Homeobox 1, HOXC6 Homeobox C6, KLK3 Kallikrein-3, SD standard deviation