Fig. 1

GAPDH expression in colorectal tumours in different stage of progression. a Representative colour photomicrographs presenting immunohistochemical staining (brown) for GAPDH in colorectal tumours (x200). Stained tissues at different stages of progression are displayed in the right column and homologous areas in negative controls in the left column. b Representative monochromatic photomicrographs were analysed with ImageJ software to quantify immunostaining and assess GAPDH expression (x150). Stained tissues at different stages of progression are portrayed in the right column and homologous areas in negative controls in the left column. For both (a) and (b), representative photomicrographs used were from sample 7058 for stage I, sample 7020 for stage II, sample 7112 for stage III and sample 7104 for stage IV