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Fig. 1 | Translational Medicine Communications

Fig. 1

From: A Solanum incanum extract (SR-T100) regresses vulvar condyloma acuminatum and induces distinct autophagic and apoptotic responses in different types of HPV-infected cells

Fig. 1

SR-T100 induced different levels of growth inhibition and apoptotic cell death in primary epithelial cells expressing different types of HPV-E6/E7. a After infection with different types Lenti-HPVE6/E7 viruses, the primary epithelial cells were treated with the indicated concentrations of SR-T100 for 24 h. Cell viability was determined using the CellTiter-Glo® luminescent cell viability assay. Each value represents the mean ± SD of three determinations. HPV-E6/E7 expression in normal cervical epithelial cells was confirmed by RT-PCR as shown in the inset panel. b HeLa cells were treated with cisplatin (150 μM) for 24 h (upper panel) and used as a positive control. After infection with different types of Lenti-HPV-E6/E7 viruses, the primary epithelial cells were treated with SR-T100 (5 μg/ml) for 12 h and double labeled with Annexin V-FITC and propidium iodide (PI). In the cytograms from three experiments, the apoptotic cells (Annexin V-positive) are distributed in the lower and upper right panels (the values are reported as percentages). c Histograms showing the number of apoptotic primary epithelial cells expressing different types of HPV-E6/E7. The results shown are the mean values of three independent experiments; bars, ±SD. ** p < 0.01, HPV6 or HPV11 versus HPV16 or HPV18

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