Fig. 2
From: Simplified assay for enrichment of primed human Th17 and Tc17 lymphocytes from peripheral blood
QRTPCR of mRNA isolated from sorted CD4 + CD146+ and CD8 + CD146+ T cells: RNA was isolated from freshly sorted lymphocytes derived from healthy individuals (n = 5), and cDNA was prepared as described and QPCRs for CD146, RORc2 and IL-17A were performed. Fold changes were normalized mRNA levels with housekeeping gene (β-actin) for (a) CD4 + CD45RO + CD146+ and (b) CD8 + CD146+ T cells were studied.compared to CD4+CD146- T cells and CD8 + CD146−T cells were studied