Fig. 4
From: Simplified assay for enrichment of primed human Th17 and Tc17 lymphocytes from peripheral blood
CD146 + CD4+ and CD8+CD146+ cells secrete significant amount of IL-17A. Lymphocytes from whole blood lysis of healthy human peripheral blood were stained with mouse anti Human mAb CD4, CD8, CD45 RO and CD146 and sorted. Sorted cells were finally washed with IMDM media and plated in 96 well flat bottom culture plate coated with purified antihuman CD3 and CD28 antibodies. Cells were incubated for 5 days and culture supernatant collected to perform multiplex using Luminex. Among analysis performed for various cytokines. Only IL-17 A was found to be significantly high among (a) CD146 + CD4+ and (b) CD146 + CD8+ cells. Samples were analyzes using Mann Whitney test and p < 0.05 was considered significant