Fig. 3

Differentially expressed proteins in the liposoluble (a) and hydrosoluble (b) fractions obtained by 2D-Dige and MALDI-TOF/MS-MS analysis from Mycoplasma fermentans MFI grown in anaerobic conditions versus aerobic. The pick number from 1 to 35 for the liposoluble fraction and 1–38 for the hydrosoluble fraction, correspond to the proteins listed in Tables 1 and 2, respectively. A cut-off value of ±1.5 was used to distinguish between proteins similarly expressed in aerobic and anaerobic conditions (− 1.5 ≤ ratio ≤ + 1.5) from proteins comparatively more expressed (ratio > + 1.5 and < − 1.5). When the same protein was found in both fractions, only the spot with higher ratio, where the protein was most represented, was included. In red are shown the proteins overexpressed in the fraction from aerobic condition, in blue are the proteins overexpressed in the fraction from anaerobic condition and in black are the proteins commonly found in both aerobic and anaerobic