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Table 1 List of proteins identified in the liposoluble fractionof Mycoplasma fermentans MFI proteome obtained from 2D page Triton X-114

From: Proteome analysis of Mycoplasma fermentans cultured under aerobic and anaerobic conditions

spot protein ID Mw (Da) pI count score Acc. No. New Acc. No. gene
1 conserved hypothetical protein / lipoprotein 10,984 9.9 4 < 95 gi|308,190,339|ref.|YP_003923270.1| WP_013355024.1 lspA
2 sucrase-isomaltase 33,221.2 7.0 6 < 95 gi|308,190,125|ref.|YP_003923056.1| WP_013354811.1 malL
3 aspartate-ammonia ligase 39,505.8 8.9 6 < 95 gi|308,189,759|ref.|YP_003922690.1| WP_013354447.1 asnA
4 excinuclease ABC subunit C 57,716.9 8.7 9 < 95 gi|308,190,195|ref.|YP_003923126.1| WP_013354881.1 uvrC
5 unidentified protein (39aa) 4542.5 10.2 5 < 95    and
6 elongation factor G 77,217.8 5.7 31 100 gi|148,377,855|ref.|YP_001256731.1 WP_011949750.1 fusA
7 DNA topoisomerase (ATP-hydrolyzing) subunit B 42,632.7 5.4 14 100 gi|308,190,314|ref.|YP_003923245.1| WP_013354999.1 parE
8 phosphoketolase family protein 89,889.5 6.4 32 100 gi|308,189,603|ref.|YP_003922534.1| WP_013354291.1 xfp
9 ATP-binding cassette domain-containing protein 47,304.9 6.0 16 100 gi|308,190,277|ref.|YP_003923208.1| WP_013354963.1 potA
10 histidine-tRNA ligase 8705.7 10.0 3 < 95 gi|308,190,116|ref.|YP_003923047.1| WP_013354802.1 hisS
11 lysine-tRNA ligase 34,794.3 9.3 12 100 gi|308,189,610|ref.|YP_003922541.1| WP_013354298.1 lysS
12–13 DNA-directed RNA polymerase alpha chain 38,023.8 5.6 23 100 gi|308,190,154|ref.|YP_003923085.1| WP_013354840.1 rpoA
14–17–19-33 elongation factor Tu (EF-Tu) 43,620.5 6.3 78 100 gi|308,189,768|ref.|YP_003922699.1| WP_013354456.1 tuf
15 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha 31,168.1 9.5 8 100 gi|308,190,218|ref.|YP_003923149.1| WP_013354904.1 pdhA
16 bifunctional oligoribonuclease/PAP phosphatase NrnA 20,844.6 6.4 8 100 gi|308,189,648|ref.|YP_003922579.1| WP_013354336.1 nrnA
18 ornithine carbamoyltransferase 38,356.7 8.0 23 100 gi|308,189,964|ref.|YP_003922895.1| WP_013354652.1 argF
20 arginine deiminase 31,623.3 5.8 14 100 gi|308,189,965|ref.|YP_003922896.1| WP_013354653.1 arcA
21 YebC/PmpR family DNA-binding transcriptional regulator 19,896.7 4.5 7 100 gi|308,189,601|ref.|YP_003922532.1| WP_013354289.1 pmpr
22–31-32 DNA polymerase III subunit gamma/tau 29,077.1 4.6 8 100 gi|308,190,298|ref.|YP_003923229.1| WP_013354984.1 dnaX
23 elongation factor P 20,893.7 5.2 8 100 gi|148,377,574|ref.|YP_001256450.1| WP_004024464.1 efp
24 HAD family phosphatase 28,122.2 7.0 15 100 gi|308,189,859|ref.|YP_003922790.1| WP_013354547.1 had
25 DNA repair protein HhH-GPD 19,566.4 7.7 6 < 95 gi|308,189,903|ref.|YP_003922834.1| WP_013354591.1 p37
26 5-dehydro-4-deoxy-D-glucuronate isomerase 31,625.1 8.2 17 100 gi|308,190,016|ref.|YP_003922947.1| WP_013354704.1 kduI
27 hyalorunate lyase 8234.2 6.1 4 < 95 gi|308,190,026|ref.|YP_003922957.1| WP_013354714.1 hysA
28 transcription elongation factor GreA 6863.6 5.1 5 100 gi|308,189,955|ref.|YP_003922886.1| WP_013354643.1 greA
29 nucleoside 2-deoxyribosyltransferase 18,332.4 5.8 12 100 gi|319,777,718|ref.|YP_004137369.1| WP_013355007.1 ndt
30–34-35 50s ribosomal protein L7/L12 12,807.9 5.1 17 100 gi|308,190,181|ref.|YP_003923112.1| WP_013354867.1 rplL
  1. The score number corresponds to the confidence of the protein ID: scores above 95% are significant; lower scores (< 95%) may be correct, but should require more evidence. The count represents the number of peptides that match the protein
  2. and not determined