Skip to main content

Table 1 List of proteins identified in the liposoluble fractionof Mycoplasma fermentans MFI proteome obtained from 2D page Triton X-114

From: Proteome analysis of Mycoplasma fermentans cultured under aerobic and anaerobic conditions

spot

protein ID

Mw (Da)

pI

count

score

Acc. No.

New Acc. No.

gene

1

conserved hypothetical protein / lipoprotein

10,984

9.9

4

< 95

gi|308,190,339|ref.|YP_003923270.1|

WP_013355024.1

lspA

2

sucrase-isomaltase

33,221.2

7.0

6

< 95

gi|308,190,125|ref.|YP_003923056.1|

WP_013354811.1

malL

3

aspartate-ammonia ligase

39,505.8

8.9

6

< 95

gi|308,189,759|ref.|YP_003922690.1|

WP_013354447.1

asnA

4

excinuclease ABC subunit C

57,716.9

8.7

9

< 95

gi|308,190,195|ref.|YP_003923126.1|

WP_013354881.1

uvrC

5

unidentified protein (39aa)

4542.5

10.2

5

< 95

  

and

6

elongation factor G

77,217.8

5.7

31

100

gi|148,377,855|ref.|YP_001256731.1

WP_011949750.1

fusA

7

DNA topoisomerase (ATP-hydrolyzing) subunit B

42,632.7

5.4

14

100

gi|308,190,314|ref.|YP_003923245.1|

WP_013354999.1

parE

8

phosphoketolase family protein

89,889.5

6.4

32

100

gi|308,189,603|ref.|YP_003922534.1|

WP_013354291.1

xfp

9

ATP-binding cassette domain-containing protein

47,304.9

6.0

16

100

gi|308,190,277|ref.|YP_003923208.1|

WP_013354963.1

potA

10

histidine-tRNA ligase

8705.7

10.0

3

< 95

gi|308,190,116|ref.|YP_003923047.1|

WP_013354802.1

hisS

11

lysine-tRNA ligase

34,794.3

9.3

12

100

gi|308,189,610|ref.|YP_003922541.1|

WP_013354298.1

lysS

12–13

DNA-directed RNA polymerase alpha chain

38,023.8

5.6

23

100

gi|308,190,154|ref.|YP_003923085.1|

WP_013354840.1

rpoA

14–17–19-33

elongation factor Tu (EF-Tu)

43,620.5

6.3

78

100

gi|308,189,768|ref.|YP_003922699.1|

WP_013354456.1

tuf

15

pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha

31,168.1

9.5

8

100

gi|308,190,218|ref.|YP_003923149.1|

WP_013354904.1

pdhA

16

bifunctional oligoribonuclease/PAP phosphatase NrnA

20,844.6

6.4

8

100

gi|308,189,648|ref.|YP_003922579.1|

WP_013354336.1

nrnA

18

ornithine carbamoyltransferase

38,356.7

8.0

23

100

gi|308,189,964|ref.|YP_003922895.1|

WP_013354652.1

argF

20

arginine deiminase

31,623.3

5.8

14

100

gi|308,189,965|ref.|YP_003922896.1|

WP_013354653.1

arcA

21

YebC/PmpR family DNA-binding transcriptional regulator

19,896.7

4.5

7

100

gi|308,189,601|ref.|YP_003922532.1|

WP_013354289.1

pmpr

22–31-32

DNA polymerase III subunit gamma/tau

29,077.1

4.6

8

100

gi|308,190,298|ref.|YP_003923229.1|

WP_013354984.1

dnaX

23

elongation factor P

20,893.7

5.2

8

100

gi|148,377,574|ref.|YP_001256450.1|

WP_004024464.1

efp

24

HAD family phosphatase

28,122.2

7.0

15

100

gi|308,189,859|ref.|YP_003922790.1|

WP_013354547.1

had

25

DNA repair protein HhH-GPD

19,566.4

7.7

6

< 95

gi|308,189,903|ref.|YP_003922834.1|

WP_013354591.1

p37

26

5-dehydro-4-deoxy-D-glucuronate isomerase

31,625.1

8.2

17

100

gi|308,190,016|ref.|YP_003922947.1|

WP_013354704.1

kduI

27

hyalorunate lyase

8234.2

6.1

4

< 95

gi|308,190,026|ref.|YP_003922957.1|

WP_013354714.1

hysA

28

transcription elongation factor GreA

6863.6

5.1

5

100

gi|308,189,955|ref.|YP_003922886.1|

WP_013354643.1

greA

29

nucleoside 2-deoxyribosyltransferase

18,332.4

5.8

12

100

gi|319,777,718|ref.|YP_004137369.1|

WP_013355007.1

ndt

30–34-35

50s ribosomal protein L7/L12

12,807.9

5.1

17

100

gi|308,190,181|ref.|YP_003923112.1|

WP_013354867.1

rplL

  1. The score number corresponds to the confidence of the protein ID: scores above 95% are significant; lower scores (< 95%) may be correct, but should require more evidence. The count represents the number of peptides that match the protein
  2. and not determined