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Table 2 List of proteins identified in the hydrosoluble fractionof Mycoplasma fermentans MFI proteome obtained from 2D page Triton X-114

From: Proteome analysis of Mycoplasma fermentans cultured under aerobic and anaerobic conditions

spot protein ID Mw (Da) pI count score Acc. No. New Acc. No. gene
1 ABC transporter ATP-binding protein 41,298.6 7.83 11 100 gi|319,777,349|ref.|YP_004137000.1| WP_013354741.1 abca
2–3–12-31-32-33-34 choline kinase 40,261.00 8.44 25 < 95 gi|308,189,726|ref.|YP_003922657.1| WP_013354414.1 licA
4 chaperone protein dnaK 58,875.1 5.43 21 100 gi|308,189,677|ref.|YP_003922608.1| WP_013354365.1 dnak
5 ATP-dependent DNA helicase PcrA 25,219.4 7.96 6 < 95 gi|308,189,989|ref.|YP_003922920.1| WP_013354677.1 pcrA
6 translation initiation factor IF-2 66,485.8 6.71 10 < 95 gi|308,189,652|ref.|YP_003922583.1| WP_013354340.1 infB
7 elongation factor G 77,217.8 5.74 24 100 gi|148,377,855|ref.|YP_001256731.1| WP_011949750.1 fusA
8–9 phosphoketolase family protein 89,889.5 6.39 57 100 gi|308,189,603|ref.|YP_003922534.1| WP_013354291.1 xfp
10 phosphoenolpyruvate--protein phosphotransferase 48,282.4 7.67 14 100 gi|308,189,969|ref.|YP_003922900.1| WP_013354657.1 ptsI
11 peptidylprolyl isomerase (trigger factor) 18,722.4 8.54 8 100 gi|308,189,700|ref.|YP_003922631.1| WP_013354388.1 tig
13 peptide chain release factor 1 39,782.6 5.40 11 100 gi|308,190,290|ref.|YP_003923221.1| WP_013354976.1 prfA
14 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha 31,168.1 9.45 13 100 gi|308,190,218|ref.|YP_003923149.1| WP_013354904.1 pdhA
15 phosphopyruvate hydratase/enolase 17,582.9 4.86 8 100 gi|308,189,766|ref.|YP_003922697.1| WP_013354454.1 eno
16 arginine deiminase 31,623.3 5.82 14 100 gi|308,189,965|ref.|YP_003922896.1| WP_013354653.1 arcA
17 elongation factor Tu (EF-Tu) 43,620.5 6.30 25 100 gi|308,189,768|ref.|YP_003922699.1| WP_013354456.1 tuf
18 ornithine carbamoyltransferase 38,356.7 8.03 23 100 gi|308,189,964|ref.|YP_003922895.1| WP_013354652.1 argF
19 DNA polymerase III subunit gamma/tau 29,077.1 4.63 3 100 gi|308,190,298|ref.|YP_003923229.1| WP_013354984.1 dnaX
20 YebC/PmpR family DNA-binding transcriptional regulator 19,896.7 4.45 7 100 gi|308,189,601|ref.|YP_003922532.1| WP_013354289.1 pmpr
21 class II fructose-1,6-bisphosphate aldolase 30,822 8.35 15 100 gi|308,189,732|ref.|YP_003922663.1| WP_013354420.1 fba
22 23S rRNA (guanosine(2251)-2′-O)-methyltransferase RlmB 25,771.6 8.89 3 < 95 gi|308,190,283|ref.|YP_003923214.1| WP_013354969.1 rlmb
23 DNA polymerase III subunit alpha 32,241.8 9.18 9 < 95 gi|308,190,227|ref.|YP_003923158.1| WP_013354913.1 dnaE
24 ribosome recycling factor 20,417.7 6.20 8 100 gi|308,190,230|ref.|YP_003923161.1| WP_013354916.1 frr
25 hyalorunate lyase 8234.2 6.13 4 < 95 gi|308,190,026|ref.|YP_003922957.1| WP_013354714.1 hysA
26 hypoxanthine phosphoribosyltransferase 14,458.7 8.78 9 100 gi|308,189,952|ref.|YP_003922883.1| WP_013354640.1 hpt
27 transcription elongation factor GreA 6863.6 5.05 5 100 gi|308,189,955|ref.|YP_003922886.1| WP_013354643.1 greA
28 nucleoside 2-deoxyribosyltransferase 18,332.4 5.78 11 100 gi|319,777,718|ref.|YP_004137369.1| WP_013355007.1 ndt
29 RpiB/LacA/LacB family sugar-phosphate isomerase 14,164.1 6.58 9 100 gi|308,189,658|ref.|YP_003922589.1| WP_013354346.1 rpiB
30 50s ribosomal protein L7/L12 12,807.9 5.08 6 100 gi|308,190,181|ref.|YP_003923112.1| WP_013354867.1 rplL
35 phosphate ABC transporter 38,776.9 8.9 6 < 95 gi|308,190,268|ref.|YP_003923199.1| WP_013354954.1 pstB
36–38 molecular chaperone DnaJ 24,597.1 9.1 13 < 95 gi|308,189,684|ref.|YP_003922615.1| WP_013354372.1 dnaJ
37 30S ribosomal protein S2 22,851 9.1 3 < 95 gi|308,189,983|ref.|YP_003922914.1| WP_013354671.1 rpsB
  1. The score number corresponds to the confidence of the protein ID: scores above 95% are significant; lower scores (< 95%) may be correct, but should require more evidence. The count represents the number of peptides that match the protein