Table 2 List of proteins identified in the hydrosoluble fractionof Mycoplasma fermentans MFI proteome obtained from 2D page Triton X-114
From: Proteome analysis of Mycoplasma fermentans cultured under aerobic and anaerobic conditions
spot | protein ID | Mw (Da) | pI | count | score | Acc. No. | New Acc. No. | gene |
|---|---|---|---|---|---|---|---|---|
1 | ABC transporter ATP-binding protein | 41,298.6 | 7.83 | 11 | 100 | gi|319,777,349|ref.|YP_004137000.1| | WP_013354741.1 | abca |
2–3–12-31-32-33-34 | choline kinase | 40,261.00 | 8.44 | 25 | < 95 | gi|308,189,726|ref.|YP_003922657.1| | WP_013354414.1 | licA |
4 | chaperone protein dnaK | 58,875.1 | 5.43 | 21 | 100 | gi|308,189,677|ref.|YP_003922608.1| | WP_013354365.1 | dnak |
5 | ATP-dependent DNA helicase PcrA | 25,219.4 | 7.96 | 6 | < 95 | gi|308,189,989|ref.|YP_003922920.1| | WP_013354677.1 | pcrA |
6 | translation initiation factor IF-2 | 66,485.8 | 6.71 | 10 | < 95 | gi|308,189,652|ref.|YP_003922583.1| | WP_013354340.1 | infB |
7 | elongation factor G | 77,217.8 | 5.74 | 24 | 100 | gi|148,377,855|ref.|YP_001256731.1| | WP_011949750.1 | fusA |
8–9 | phosphoketolase family protein | 89,889.5 | 6.39 | 57 | 100 | gi|308,189,603|ref.|YP_003922534.1| | WP_013354291.1 | xfp |
10 | phosphoenolpyruvate--protein phosphotransferase | 48,282.4 | 7.67 | 14 | 100 | gi|308,189,969|ref.|YP_003922900.1| | WP_013354657.1 | ptsI |
11 | peptidylprolyl isomerase (trigger factor) | 18,722.4 | 8.54 | 8 | 100 | gi|308,189,700|ref.|YP_003922631.1| | WP_013354388.1 | tig |
13 | peptide chain release factor 1 | 39,782.6 | 5.40 | 11 | 100 | gi|308,190,290|ref.|YP_003923221.1| | WP_013354976.1 | prfA |
14 | pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha | 31,168.1 | 9.45 | 13 | 100 | gi|308,190,218|ref.|YP_003923149.1| | WP_013354904.1 | pdhA |
15 | phosphopyruvate hydratase/enolase | 17,582.9 | 4.86 | 8 | 100 | gi|308,189,766|ref.|YP_003922697.1| | WP_013354454.1 | eno |
16 | arginine deiminase | 31,623.3 | 5.82 | 14 | 100 | gi|308,189,965|ref.|YP_003922896.1| | WP_013354653.1 | arcA |
17 | elongation factor Tu (EF-Tu) | 43,620.5 | 6.30 | 25 | 100 | gi|308,189,768|ref.|YP_003922699.1| | WP_013354456.1 | tuf |
18 | ornithine carbamoyltransferase | 38,356.7 | 8.03 | 23 | 100 | gi|308,189,964|ref.|YP_003922895.1| | WP_013354652.1 | argF |
19 | DNA polymerase III subunit gamma/tau | 29,077.1 | 4.63 | 3 | 100 | gi|308,190,298|ref.|YP_003923229.1| | WP_013354984.1 | dnaX |
20 | YebC/PmpR family DNA-binding transcriptional regulator | 19,896.7 | 4.45 | 7 | 100 | gi|308,189,601|ref.|YP_003922532.1| | WP_013354289.1 | pmpr |
21 | class II fructose-1,6-bisphosphate aldolase | 30,822 | 8.35 | 15 | 100 | gi|308,189,732|ref.|YP_003922663.1| | WP_013354420.1 | fba |
22 | 23S rRNA (guanosine(2251)-2′-O)-methyltransferase RlmB | 25,771.6 | 8.89 | 3 | < 95 | gi|308,190,283|ref.|YP_003923214.1| | WP_013354969.1 | rlmb |
23 | DNA polymerase III subunit alpha | 32,241.8 | 9.18 | 9 | < 95 | gi|308,190,227|ref.|YP_003923158.1| | WP_013354913.1 | dnaE |
24 | ribosome recycling factor | 20,417.7 | 6.20 | 8 | 100 | gi|308,190,230|ref.|YP_003923161.1| | WP_013354916.1 | frr |
25 | hyalorunate lyase | 8234.2 | 6.13 | 4 | < 95 | gi|308,190,026|ref.|YP_003922957.1| | WP_013354714.1 | hysA |
26 | hypoxanthine phosphoribosyltransferase | 14,458.7 | 8.78 | 9 | 100 | gi|308,189,952|ref.|YP_003922883.1| | WP_013354640.1 | hpt |
27 | transcription elongation factor GreA | 6863.6 | 5.05 | 5 | 100 | gi|308,189,955|ref.|YP_003922886.1| | WP_013354643.1 | greA |
28 | nucleoside 2-deoxyribosyltransferase | 18,332.4 | 5.78 | 11 | 100 | gi|319,777,718|ref.|YP_004137369.1| | WP_013355007.1 | ndt |
29 | RpiB/LacA/LacB family sugar-phosphate isomerase | 14,164.1 | 6.58 | 9 | 100 | gi|308,189,658|ref.|YP_003922589.1| | WP_013354346.1 | rpiB |
30 | 50s ribosomal protein L7/L12 | 12,807.9 | 5.08 | 6 | 100 | gi|308,190,181|ref.|YP_003923112.1| | WP_013354867.1 | rplL |
35 | phosphate ABC transporter | 38,776.9 | 8.9 | 6 | < 95 | gi|308,190,268|ref.|YP_003923199.1| | WP_013354954.1 | pstB |
36–38 | molecular chaperone DnaJ | 24,597.1 | 9.1 | 13 | < 95 | gi|308,189,684|ref.|YP_003922615.1| | WP_013354372.1 | dnaJ |
37 | 30S ribosomal protein S2 | 22,851 | 9.1 | 3 | < 95 | gi|308,189,983|ref.|YP_003922914.1| | WP_013354671.1 | rpsB |