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Table 1 Summary of characteristics of various CD4+ T cells enumeration techniques

From: Performance evaluation of the novel portable PIMA point-of-care CD4 analyzer in comparison with conventional flow cytometer instruments in Cameroon

Parameter Dedicated technology based assay
  FACSCalibur FACSCount CyFlow PIMA
Manufacturer Becton Dickinson (CA, USA) Becton Dickinson (CA, USA) Partec GmbH (Munster,Germany) Alere Medical Pvt. Ltd., USA
Platform TruCount Dedicated CD4/CD4% counter Dedicated CD4 Count Dedicated CD4 Count
Principle Flow cytometry Flow cytometry Flow cytometry Flow cytometry
Monoclonal antibodies used Multitest CD3/CD8/CD45/CD4 Anti-CD4 &anti CD3 Anti-CD3, anti-CD4 Anti-CD3, anti-CD4
Cost per test (US$) 30 25 12 10
Cost of the instrument (US$) 75,000 30,000 22,000 12,000
Specimen type whole blood whole blood whole blood whole blood
specimen volume (μL) 50 50 50 25
Technique Lyse no wash No lyse No wash No lyse No wash No lyse No wash
Gating strategy CD45/SSC CD3/SSC partec gating CD3
Structure CIRCB (a) GHY (b) Day Hospital CHY© CIRCB, GHY, CHY
  1. (a) “Chantal BIYA International Reference Centre for Research on Prevention and Management of HIV/AIDS (CIRCB)
  2. (b) General Hospital of Yaounde
  3. © Day Hospital Central Hospital of Yaounde (CHY)