Parameter | Dedicated technology based assay | |||
---|---|---|---|---|
FACSCalibur | FACSCount | CyFlow | PIMA | |
Manufacturer | Becton Dickinson (CA, USA) | Becton Dickinson (CA, USA) | Partec GmbH (Munster,Germany) | Alere Medical Pvt. Ltd., USA |
Platform | TruCount | Dedicated CD4/CD4% counter | Dedicated CD4 Count | Dedicated CD4 Count |
Principle | Flow cytometry | Flow cytometry | Flow cytometry | Flow cytometry |
Monoclonal antibodies used | Multitest CD3/CD8/CD45/CD4 | Anti-CD4 &anti CD3 | Anti-CD3, anti-CD4 | Anti-CD3, anti-CD4 |
Cost per test (US$) | 30 | 25 | 12 | 10 |
Cost of the instrument (US$) | 75,000 | 30,000 | 22,000 | 12,000 |
Specimen type | whole blood | whole blood | whole blood | whole blood |
specimen volume (μL) | 50 | 50 | 50 | 25 |
Technique | Lyse no wash | No lyse No wash | No lyse No wash | No lyse No wash |
Gating strategy | CD45/SSC | CD3/SSC | partec gating | CD3 |
Structure | CIRCB (a) | GHY (b) | Day Hospital CHY© | CIRCB, GHY, CHY |