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Table 1 Summary of characteristics of various CD4+ T cells enumeration techniques

From: Performance evaluation of the novel portable PIMA point-of-care CD4 analyzer in comparison with conventional flow cytometer instruments in Cameroon

Parameter

Dedicated technology based assay

 

FACSCalibur

FACSCount

CyFlow

PIMA

Manufacturer

Becton Dickinson (CA, USA)

Becton Dickinson (CA, USA)

Partec GmbH (Munster,Germany)

Alere Medical Pvt. Ltd., USA

Platform

TruCount

Dedicated CD4/CD4% counter

Dedicated CD4 Count

Dedicated CD4 Count

Principle

Flow cytometry

Flow cytometry

Flow cytometry

Flow cytometry

Monoclonal antibodies used

Multitest CD3/CD8/CD45/CD4

Anti-CD4 &anti CD3

Anti-CD3, anti-CD4

Anti-CD3, anti-CD4

Cost per test (US$)

30

25

12

10

Cost of the instrument (US$)

75,000

30,000

22,000

12,000

Specimen type

whole blood

whole blood

whole blood

whole blood

specimen volume (μL)

50

50

50

25

Technique

Lyse no wash

No lyse No wash

No lyse No wash

No lyse No wash

Gating strategy

CD45/SSC

CD3/SSC

partec gating

CD3

Structure

CIRCB (a)

GHY (b)

Day Hospital CHY©

CIRCB, GHY, CHY

  1. (a) “Chantal BIYA International Reference Centre for Research on Prevention and Management of HIV/AIDS (CIRCB)
  2. (b) General Hospital of Yaounde
  3. © Day Hospital Central Hospital of Yaounde (CHY)