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Fig. 1 | Translational Medicine Communications

Fig. 1

From: Cell Banking of HEK293T cell line for clinical-grade lentiviral particles manufacturing

Fig. 1

Layout of Creatio GMP facility. The facility is controlled by three independent Heating Ventilation and Air Conditioning (HVAC) systems (orange, blue and green). In white, non-classified areas. In orange, HVAC-1. In blue, HVAC-2. In green, HVAC-3. Rooms belonging to HVAC-3 have a pressure of 10 Pa and are dedicated to storage and product conditioning. Cell therapy products are manufactured in HVAC-1. Cleanrooms (Grade B) belonging to HVAC-1 have a pressure of 50 Pa, adjacent rooms have a differential pressure of 10–15 Pa. HVAC-2 is dedicated to gene therapy products. Access to gene therapy cleanrooms (Grade B) is granted through dressing rooms which have a differential pressure of 10–15 Pa. In this case, Grade B cleanroom is under pressured (30 Pa) compared to the adjacent dressing room (40 Pa) as a physical containment barrier to avoid viral spreading

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