Skip to main content
Fig. 2 | Translational Medicine Communications

Fig. 2

From: Cell Banking of HEK293T cell line for clinical-grade lentiviral particles manufacturing

Fig. 2

MCB workflow. MCB was composed of 3 independent batches containing 20 or more cryotubes. The HEK293T cell line cryovial was thawed and expanded until reaching 200·106 cells. Two cell passages were needed to obtain the final number of 200·106 cells. After the second passage, cells were transferred in cryotubes at a concentration of 10·106 cells per tube and frozen using a ramp program that lowers the temperature 1 °C/minute. MCB cryotubes were transferred to a gas phase nitrogen tank at -180 °C ± 20° C. WCB was composed of 3 independent batches containing 50 or more cryotubes. Environmental controls, IPCs and quality control test results are shown in the image. b WCB workflow. MCB HEK293T were thawed and expanded to reach 1000·106 cells. Only one cell passage was needed to obtain 1000·106 cells. Cells were transferred into cryotubes at a concentration of 20·106 cells per tube and frozen using a ramp program that lowers the temperature 1 °C/minute. WCB cryotubes were transferred to a gas phase nitrogen tank at -180 °C ± 20° (C) Environmental controls, IPCs and quality control test results are represented in the image

Back to article page