Table 1 Primer sequence used for detection of ACE rs4646994, AGT rs699 and AGTR1 rs5186 gene polymorphisms
Polymorphisms | Sequence (5`- > 3`) | Restriction Enzyme | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
ACE rs4646994 | F: GCCCTGCAGGTGTCTGCAGCATGT R: GGATGGCTCTCCCCGCCTTGTCTC | – | I allele: 599 bp D allele: 312 bp | 66 |
AGT rs699 | F: CCGTTTGTGCAGGGCCTGGCTCTCT R: CAGGGTGCTGTCCACACTGGACCCC | Tth111I | T allele: 165 C allele: 141 + 24 | 68 |
AGTR1 rs5186 | F: AGAAGCCTGCACCATGTTTTGAG R: CCTGTTGCTCCTCTAACGATTTA | DdeI | A allele: 410 bp C allele: 292 + 118 | 60 |