Fig. 1
Flowchart showing the study design and subsequent division of samples into groups for further analysis. Samples were collected and processed, after which ultra performance liquid chromatography-ultra definition mass spectrometry (UPLC-UDMSE) was performed. The raw data was analyzed and we subsequently identified 361 proteins with two or more unique peptides. The samples were divided into groups, after which further statistical analyses were performed. First, the samples were divided into three groups: women who developed PE (“Pre-eclampsia”), women with clinical risk factors for PE who did not develop PE and were classified as at-risk controls (“Risk controls”), and controls without known risk factors for PE (“Healthy controls”). Within each group, the samples were also analyzed according to time point when the samples were obtained (12–14 weeks, 18–20 weeks, or 26–28 weeks of gestation)