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Fig. 1 | Translational Medicine Communications

Fig. 1

From: Imaged-guided focused ultrasound in combination with various formulations of doxorubicin for the treatment of diffuse intrinsic pontine glioma

Fig. 1

Experimental design. BBB opening after FUS was determined in HSJD-DIPG-07 tumor-bearing mice which received 200 kPa (n = 3) and 400 kPa (n = 3) to observe the extent of BBB opening. Mice received 400 kPa during treatment in all subsequent experiments. Survival analysis was performed on HSJD-DIPG-07 xenograft models (n = 54). Mice were regularly monitored with BLI and upon increase of the signal, mice received treatment with focused ultrasound. After treatment, mice were monitored and sacrificed at humane endpoints. Immunofluorescence histology was performed on non-tumor bearing mice (n = 3) and tumor bearing mice (HSJD-DIPG-07) (n = 6) to determine the presence of liposomal formulations of doxorubicin in the brain parenchyma. At 24 h after treatment mice were sacrificed and brains were frozen until analysis. (Created with Biorender)

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