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Fig. 5 | Translational Medicine Communications

Fig. 5

From: Targeted quantitation of CFTR protein expression in vivo using immunoprecipitation & parallel reaction monitoring tandem mass spectrometry

Fig. 5

Comparison of MS responses of five hCFTR peptides (AVQPLLLGR, GQLLAVAGSTGAGK, LSLVPDSEQGEAILPR, NSILNPINSIR, and SVIEQFPGK) generated by IP using 8 different anti-human CFTR antibodies. hCFTR protein (1 ng) spiked in mouse lung membrane protein extracts (25 mg lung tissue) was used as test sample. Protein G magnetic beads and un-biotinylated antibody were used for IP. Data were processed by skyline software. Anti-human CFTR antibody information was shown in Table S2. The reproducible result was shown in Figure S4

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Translational Medicine Communications

ISSN: 2396-832X

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