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Fig. 6 | Translational Medicine Communications

Fig. 6

From: Targeted quantitation of CFTR protein expression in vivo using immunoprecipitation & parallel reaction monitoring tandem mass spectrometry

Fig. 6

Standard curve of hCFTR by plotting peak area ratio of light to heavy peptide LSLVPDSEQGEAILPR in mouse lung membrane protein extracts. Streptavidin magnetic beads and biotinylated anti-CFTR antibody were used for IP. hCFTR concentration was 0.5, 1.4, 4.1, 12, 37, to 111 pg per mg tissue for standard 1 to 6, respectively. Calibration curve in linear regression with weighting 1/X was plotted by using Xcalibur software

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Translational Medicine Communications

ISSN: 2396-832X

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