Fig. 3From: Targeting EphA2 and DDR signaling can overcome the BRAF and MEK inhibitors acquired resistance in melanoma cell lines Expression and activation of receptor tyrosine kinases (RTK) and sensitivity to ALW‑II‑41‑27inhibitor in parental and resistant melanoma cell lines. A Cell lysates of SAN, SAN-VRCR, A375 and A375-VRCR cell lines were analyzed using the TKR array kit. The graph represents quantification of mean pixel density using ImageJ program. B Western blot analysis of EphA2, DDR1 and DDR2 proteins in basal condition of melanoma cancer cell lines. Tubulin was used as a loading control. C Cell viability was evaluated by MTT staining on parental (SAN and A375) and resistant (SAN-VRCR and A375-VRCR) cell lines following treatment for 72 h with increasing concentrations of ALW‑II‑41‑27inhibitor (range 0,01–10 µM). D Western blot analysis of EphA2, DDR1, DDR2, AKT, MAPK and MEK proteins in all cell lines untreated and treated with ALW‑II‑41‑27 inhibitor (0,5 µM)Back to article page