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Fig. 3 | Translational Medicine Communications

Fig. 3

From: Targeting EphA2 and DDR signaling can overcome the BRAF and MEK inhibitors acquired resistance in melanoma cell lines

Fig. 3

Expression and activation of receptor tyrosine kinases (RTK) and sensitivity to ALW‑II‑41‑27inhibitor in parental and resistant melanoma cell lines. A Cell lysates of SAN, SAN-VRCR, A375 and A375-VRCR cell lines were analyzed using the TKR array kit. The graph represents quantification of mean pixel density using ImageJ program. B Western blot analysis of EphA2, DDR1 and DDR2 proteins in basal condition of melanoma cancer cell lines. Tubulin was used as a loading control. C Cell viability was evaluated by MTT staining on parental (SAN and A375) and resistant (SAN-VRCR and A375-VRCR) cell lines following treatment for 72 h with increasing concentrations of ALW‑II‑41‑27inhibitor (range 0,01–10 µM). D Western blot analysis of EphA2, DDR1, DDR2, AKT, MAPK and MEK proteins in all cell lines untreated and treated with ALW‑II‑41‑27 inhibitor (0,5 µM)

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