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Fig. 1 | Translational Medicine Communications

Fig. 1

From: Circulating neutrophil extracellular trap (NET)-forming ‘rogue’ neutrophil subset, immunotype [DEspR + CD11b +], mediate multi-organ failure in COVID-19—an observational study

Fig. 1

Representative images of differential levels of circulating NET + Ns by immunofluorescence-cytology (IFC). A-B Representative IFC confocal microscopy image [z-stack through nucleus] showing minimal to no DEspR + CD11b + [NET + Ns] with extruded DNA (blue) in a (A) COVID-19 survivor, and (B) increased DEspR + [NET + Ns] in COVID-19 non-survivor. Bar 10 μm; DEspR + CD11b + microvesicles observed < 1 μm. Panel A #1 and Panel B #2: neutrophils shown in Fig. 2-A. C Representative IFC images of DEspR + [NET + Ns] showing from left to right: DAPI-DNA staining of decondensing nuclei in various stages of extrusion of DAPI-stained DNA, citrullinated histone-3 (citH3) expression with varying intensities, neutrophil DEspR + expression, merged image, and merged image with differential interference contrast (DIC) superimposed. Bar = 10 microns

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Translational Medicine Communications

ISSN: 2396-832X

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