Individualized body bioelectrical impedance parameters in newly diagnosed cancer children

The bioelectric impedance analysis permits to estimate electrical parameters and body composition of subjects who are either apparently healthy or sick with different pathologies. The aim of this study is to individualize the analysis of body bioelectrical impedance parameters in newly diagnosed cancer children, by means of the bioelectrical impedance analysis for each age group, gender and cancer histological variety. This retrospective cross-sectional study consisted of 43 pediatric patients with different histological varieties of cancer, ages from 2 to 17. The body electrical resistance and body capacitive electrical reactance were measured with the Bodystat 1500-MDD analyzer. From these two electrical parameters the body electrical impedance modulus and the body phase angle were calculated. The results showed that 93.02% of cancer children were outside reference rectangles according to age groups and gender were showed. The values of body capacitive electrical reactance (72.5%) and body phase angle (90.70%) of these patients were below the lower limits of their respective rectangles. These findings were noticeable for patients who had solid tumors. The BIA is feasible to individualize body bioelectrical parameters and body bioelectric state in newly diagnosed cancer children and how differ from those in apparently healthy subjects, for the same age group and gender. Additionally, the tumor electrical properties may have a noticeable role in changes of body bioelectric-physiological parameters of these newly diagnosed cancer children.


Background
The malignant tumor is the second cause of morbidity and mortality in Cuba and worldwide. The number of cancer patients is expected to increase progressively in the next years [1]. In Cuba, cancer is the first cause of death for patients ages 5 to 15 and the second cause of death in children between 1 and 4 years old [2]. These statistics are similar to those reported in other countries [1]. Around 400 children are diagnosed each year in Cuba and 35 of them correspond approximately to Santiago de Cuba province. This number has increased to 60 in the last triennium [2]. Acute lymphoblastic leukemia, central nervous system tumors, lymphomas, bone tumors and renal tumors are the most common malignant neoplasms in pediatric patients (1-18 years old) [2][3][4].
Cancer in pediatric patients can be diagnosed by clinical method and laboratory, pathologic and imaging tests [5]. Additionally, electrical impedance tomography, diffusion tensor magnetic resonance imaging, magnetic resonance electrical impedance tomography, magnetic induction tomography, magnetoacoustic tomography, Hall effect imaging and magnetoacoustic tomography with magnetic induction techniques have been suggested to electrically differentiate cancer from its surrounding healthy tissue based on differences between their electrical properties [6]. Tissue electrical properties have also been used to estimate the body composition, body water, among others in subjects who are either apparently healthy or sick with different pathologies by means of the bioelectric impedance analysis (BIA) [7,8].
The BIA permits the estimation of different body bioelectric parameters, such as the body electrical resistance (R), body capacitive electrical reactance (Xc), body electrical impedance modulus (|Z|) and body phase angle (θ). Parameters R and Xc have been related to the body hydration status and body cell mass of subjects who are either apparently healthy or sick with different pathologies. These relationships have been established by means of different estimation equations [9][10][11][12]. The parameter θ is associated with the pathology severity and the quality of life, survival and prognosis of the evolution of a patient [8,[13][14][15]. In addition, BIA has been used to establish the normal intervals (means ± standard deviations) of R, R/H, Xc, Xc/H and θ of different populations of apparently healthy subjects in different countries [16,17]. The variables H, R/H and Xc/H are the height of the subject, the body electrical resistance per unit of height and the body capacitive electrical reactance per unit of height, respectively.
Normal intervals of R/H, Xc/H and θ for a population of apparently healthy subjects of Santiago de Cuba, Cuba, by gender and age groups (2-80) are reported in [16]. For this, the BioScan 98 BIA analyzer (Biológica Tecnología Médica SL, Barcelona, Spain, available at http://www.blbiologico.es) is used. In addition, the Bland-Altman statistical method is used to assess the agreement between the BioScan 98 BIA analyzer and the Bodystat 1500-MDD BIA analyzer (Bodystat Firm Inc., Tampa, Florida, USA, available at http://www.bodystat.com) [18], as suggested in other studies [19,20]. Values of R, Xc and θ generated with two devices are equals when correction factors for R and Xc are introduced in the BioScan 98 (+ 14.7 Ω for R and + 22 Ω for Xc) and Bodystat 1500 MDD (− 3 Ω for R and + 9 Ω for Xc) BIA analyzers, as demonstrated in [18]. Consequently, these two BIA analyzers may be suitable in clinics. When the corrected and uncorrected R and Xc values are entered in any of total body water estimation equations, non-significant differences are reported from the statistical and clinical points of view [21]. This finding has been also observed for the fat free mass when the corrected and uncorrected R and Xc values are introduced in each one their estimation equations [22]. In these two studies, both BioScan 98 and Bodystat 1500-MDD BIA analyzers are used to estimate these values of R and Xc.
It is well documented in the literature that the bivariate analysis is one of the simplest forms of statistical analysis. It consists of the relationships between pairs of variables in a data set. This bivariate analysis usually involves the variables X and Y [23,24]. Bivariate statistics has been extended to different problems of the science [25], specifically in the area of the bioelectrical impedance, known as the bioelectrical impedance vector analysis (BIVA). Piccoli is a pioneer of BIVA method [8,[26][27][28].
In BIVA plot, the variables X and Y are R/H (R) and Xc/H (Xc), respectively. Additionally, the reference intervals for any individual are represented in BIVA plots as 50, 75 and 95% tolerance ellipses obtained from apparently healthy population [8,28]. This idea has been extended to other BIA studies [16,29,30].
Although BIA has been used in pediatric patients with cancer [14,[32][33][34][35], we are not aware of its use to differentiate bioelectric parameters of newly diagnosed cancer children or untreated cancer children with those of apparently healthy population, by gender, age group and cancer type. The aim of this study is to individualize the analysis of body bioelectrical impedance parameters in newly diagnosed cancer children, by means of the bioelectrical impedance analysis for each age group, gender and cancer histological variety. Values of R/H, Xc/H and θ of patients with solid tumor (Ts) and non-solid tumor (Tns) are compared with those estimated in apparently healthy pediatric subjects reported in [16]. Pediatric patients were included in this research once their carers (parents, family or person in custody) read, agreed and signed the written Informed Consent. This written Informed Consent was signed by the parent/ guardian, medical oncologist and a psychologist (as a witness). It is important to note that prior to the signing of the written Informed Consent, aims, importance and purposes of this investigation were explained to carers, as well as all requirements for the measurements. These requirements were empty bladder, 12 h of fasting and 12 h of no physical exercises before measurements.

Sample characteristics
The inclusion criteria were the free consent of carers, the children with cancer at any stage confirmed by pathological anatomy and oncological patients at the Oncology and Hematology department in the Hospital Infantil Sur Antonio María Béguez César. The exclusion criteria were carers/patients who did not wish to participate in the research and/or children had significant alterations, such as amputees, with generalized skin diseases, serious infections, symptomatic congestive heart failure, body fluid disorders and hemostatic. The interruption criteria were voluntary abandonment and death of the cancer patient.
The sample consisted of 43 untreated cancer children, ages 2 to 17. These patients were divided into two experimental groups. The first formed by 25 patients with different histological varieties of Ts and the second consisted of 18 patients with various types of Tns. The individual analysis of each patient was performed by each age group and gender, taking into account [16]. Each patient was assigned a numerical code from 1 to 43.

Measurement procedure with the Bodystat 1500-MDD BIA analyzer
The Bodystat 1500-MDD BIA analyzer was used to estimate R (in Ω) and Xc (in Ω) in patients with Ts and Tns. Values of |Z| (in Ω) and θ (in o ) were calculated from R and Xc, using the expressions and θ = tg −1 (Xc/R), respectively. In this study, |Z| was not reported because its values were similar to those of R and their contributions on θ were also comparable. Maximum difference between R and |Z| values in cancer patients was ≤3 Ω. Additionally, maximum difference between θ values was ≤0.01 o when R and |Z| are considered on the mathematical expression for θ. These differences had no significance in the clinical order, as in [18,21,22].
Although this device operated at frequencies of 5 and 50 kHz, the body values of R, Xc, |Z| and θ were reported only at 50 kHz. The amplitude of the sinusoidal electric current applied was 800 μA. Additionally, the calibrator (500.0 ± 0.1 Ω) was supplied by the manufacturer of the Bodystat 1500-MDD BIA analyzer and used to assess the stability of this device at the beginning and at the end of measurement in each patient.
This study was governed by the same methodology established in [18,21,22] and recommendations established by the National Institutes of Health Technology Assessment Conference Statement for the measurement of bioelectrical parameters [37]. For the measurement of R p and Xc p values (the subscript p was referred to the cancer patient), the following methodology was followed [18,21,22]. First, H and the weight of cancer patient were measured with a medical mechanical scale with stadiometer (model ZT-120, Zheiang, China) of accuracy ±0.1. The minimum value of weight per division was 0.5 Kg and the minimum value height per division was 0.5 cm.
Second, patients covered with light clothing were placed in the supine position on a non-conductive surface, without a pillow under the head, with the arms separated at approximately 30°from the thorax and the legs separated approximately at an angle of 45°without contact between them. The skin of each patient was first cleaned with water and soap, and then 70% alcohol.
Third, the stimulating electrodes (or injection electrodes of alternating electric current) were placed in the medial areas of the dorsal surfaces of hands and feet, near the metacarpal and metatarsal phalangeal joints, respectively. The sensing electrodes (or receiving electrodes the body electrical voltage) were placed between the distal epiphyses of the radius and the ulna, at the level of the pisiform eminence, as well as at the midpoint between both malleoli, respectively. The distance between the sensing and stimulating electrodes was 5 cm and measured with a standard measuring tape (Lotus model, Ningbo Sunshine Company, Ningbo, China) of 0.1 cm precision. Electrocardiogram electrodes (model APR-020, All Pro Corporation Company, Quingdao, China) were used. The material of each electrode was Silver/Silver Chloride (Ag/AgCl).
Fourth, R p and Xc p measurements were carried out in controlled environmental conditions of temperature (25.0 ± 1.0°C), relative humidity (60 to 65%) and free environment of devices generating field and electromagnetic radiation. These two physical quantities were measured with a digital humidity and relative temperature meter Testo (model 608-H1, Shanghai, China). The accuracy of this instrument was ±0.5°C and ± 3% for temperature and relative humidity, respectively. In addition, R p and Xc p measurements in each patient were between 8 and 9 am, by a previously trained nurse.

Comparison between apparently healthy and sick subjects
Average values of (R/H) r , (Xc/H) r and θ r were taken as references. This was argued because BioScan 98 and Bodystat 1500-MDD BIA analyzers were electrically equivalent, as demonstrated in [18]. The subscript r was referred to the apparently healthy subjects. This population was used as reference. The means ±2 standard deviations of (R/H) r , (Xc/H) r and θ r of apparently healthy children by age group and gender were reported in [16]. These ranges were displayed in Table 1 to facilitate the reading of this study. Data shown in this table were provided by MSc. Alcibiades Lara Lafargue (researcher in charge of the population study in apparently healthy subjects). The latter was conducted at the Centro Nacional de Electromagnetismo Aplicado, Universidad de Oriente in Santiago de Cuba, Cuba (this institution will keep the original data of this population study for 15 years, according to the regulations established by the Ministry of Public Health of the Republic of Cuba).
The interval 17-59 was added in this study because a cancer infant was 17 years old (Table 1) This comparison allowed us to know if a population of pediatric patients with cancer differed from that of the apparently healthy subjects, for each age group, gender and experimental group.
Since the Xc/H versus R/H plot was a twodimensional Euclidean space, the ordered pair (R/H, Xc/ H) was interpreted as the body bioelectric state of any subject from the bioelectrical point of view. In this study, body bioelectric states of the apparently healthy subject were represented by the ordered pair ((R/H) r , (Xc/H) r ). Body bioelectric states of the patient with Ts/Tns were denoted by the ordered pair ((R/H) p , (Xc/H) p ). Additionally, the body bioelectric state of each apparently healthy subject could be symbolized by the ordered pair (R r , Xc r ). The body bioelectric state of each untreated cancer pediatric patient could be represented by the ordered pair (R r , Xc r ). These two later asseverations were argued because the measurement was made in the same subject.

Statistical analysis
Spearman's rank correlation coefficient (or Spearman's rho) and Pearson correlation were used to know the lineal correlation between two variables: R p and Xc p , (R/H) p and (Xc/H) p , θ p and R p , θ p and Xc p , θ p and (R/H) p , and θ p and (Xc/H) p for the solid and non-solid tumors. Probability (pvalue) was associated to each correlation analysis. Lineal correlation was significant when p < 0.05 (significance level).

Euclidean distance
The Euclidean distance, named d pr (Ω/m), between two points ((R/H) p , (Xc/H) p ) and ((R/H) r , (Xc/H) r ) was calculated by means of the following equation In eq. (1), d pr was calculated taking into account the gender, age and experimental group.
As ((R/H) r , (Xc/H) r ) represented the central point of the reference rectangle and ((R/H) p , (Xc/H) p ) the bioelectrical state of any cancer child for each gender and age group, their impedance vectors referred to coordin- It should be noted that the angle between Z ! r and Z [23]. From the mathematical point of view, d pr denoted the ordinary distance between two points in the plane Xc/ H-R/H. Nevertheless, from the bioelectric point of view, d pr meant how different the body bioelectric state of a cancer child was with respect to that of a reference population, for the same age group and gender. In other words, d pr was a measure of how the body bioelectric state of pediatric patient was affected by the presence of Ts/Tns. From the biophysical-chemical-energetic-clinical points of view, an increase in d pr was related to a greater degradation, smaller survival and quality of life of newly diagnosed cancer children.   Table 3. For both tumor types, similar correlation coefficients were reported when the analysis included R p and Xc p , θ p and R p and θ p and Xc p .
The code, cancer histological variety, age, gender, height and values of R p , (R/H) p , Xc p , (Xc/H) p , θ p and d pr for each patient with Ts and Tns were showed in Tables 2 and 3, respectively. R p , (R/H) p , Xc p , (Xc/H) p and θ p were used to refer the values of R, R/H, Xc, Xc/H and θ for these patients. 58.14% (25/43) corresponded to patients with Ts and 41.86% (18/43) related to patients with Tns were revealed in these two tables. In addition,  For each gender and age group shown in Table 1, all possible combinations of (R/H) r and (Xc/H) r for a population of apparently healthy pediatric subject were included in delimited rectangles with continuous and dashed lines for the male and female genders, respectively (Fig. 1). This meant that body bioelectric-physiological states of these subjects were only possible if they belonged to the interior and contour of their respective rectangles. In other words, these states were not possible outside of them. R/H and Xc/H values outside the rectangle represented a subject with some type of pathology (for example, cancer patients), for each gender and age group. Therefore, in this study, this rectangle for apparently healthy subjects was named the reference rectangle for body bioelectric states (R r ). The normal intervals for each rectangle displayed in Fig. 1 were made using ±2 standard deviations of reference source data [16], as displayed in Table 1. In Fig. 1, the center circle in R r delimited with a continuous line named (R rc ) represented the ordered pair ((R/H) r , (Xc/H) r ) for the male gender. The unfilled triangle in the center of the R r delimited with dashed line, named R rd , symbolized the ordered pair ((R/H) r , (Xc/ H) r ) for the female gender. In addition, R rc , R rd and the body bioelectric state of each male and female patient with Ts and Tns were displayed in this figure for 2-3 years (Fig. 1a), 4-5 years (Fig. 1b), 6-7 years (Fig. 1c), 8-9 years (Fig. 1d), 10-11 years (Fig. 1e), 12 years (Fig. 1f), 13-16 years (Fig. 1g) and 17-59 years (Fig. 1h). Additionally, Fig. 1  In Fig. 2, R rc and R rd of all apparently healthy pediatric subjects were shown. The set of these R rc and R rd defined the entire region in which were most likely present all bioelectric states of them. In addition, the body bioelectric state of each male and female cancer patient was illustrated in this figure in order to know if the body bioelectric state of the pediatric patient depended on gender, age group and tumor histological variety. Figure 2 revealed that 55.81% (24/43) of body bioelectric states of cancer patients were distributed in R rc or their respective neighborhoods, by gender, age group and experimental group. The body bioelectric states of the remaining patients 44.19% (19/43) were distributed regardless of gender and age group. These findings were observed in 60.00% (15/25) and 50.00%

Discussion
The results of this study are valid for the research period and the number of patients who fulfill the inclusion criteria. These inclusion criteria and low prevalence of cancer children in the province of Santiago de Cuba may explain why the sample size of this study is lower. In addition, the race of cancer children in this study is the same than the one reported in [16] because the ethnography of Santiago de Cuba population is characterized by a mixture of several race ethnicities (European whites, aboriginal Indians and African blacks). Nevertheless, the gender ratio of cancer children is different to that reported in [16]. This difference may be explained because gender ratio can be controlled/fixed in a population study, but not in a study with cancer patients. Gender ratio of cancer patients depends on disease prevalence for gender and age group, and inclusion, exclusion and interruption criteria. On the other hand, results of this study confirm that acute lymphoid leukemia, non-Hodgkin lymphoma and cancer of the central nervous system are most frequently observed in untreated pediatric patients, as in [2-4, [38][39][40][41]. Additionally, this study confirms that acute lymphoid leukemia is more frequent than acute myeloid leukemia, in agreement with [39,41]. The predominance of the male gender of these patients confirms the result documented in [42] and contradicts that reported by Toquica et al. [41].
For both tumor types, p-values indicated that correlation between θ p and (R/H) p was non-significant and therefore non-lineal. Nevertheless, significant, lineal and low correlations were reported for ((R/H) p and (Xc/H) p ) and (θ p and (Xc/H) p ).
Several reasons can be attributed to nearly 10% of cancer patient not satisfying the criteria of phase angle, such as: the biological individuality, the phase angle does not have to change once the cancer patients is diagnosed for the first time, not all new diagnosed cancer children have the same performance status and detected at the same time and not all tumor type influences equally θ p . These reasons justify why the individual analysis of the bioelectrical parameters is suggested in this study.
The finding that 90.70% of all cancer patients satisfy θ p < θ r confirms that the phase angle may be used as an indicator of disease severity and quality of life and loss of body homeostasis of the cancer patient, a matter that agrees with [14]. These findings have been confirmed in adult patients with cancer [8,42,43] and in patients with other pathologies [8,13,15,34,44]. Gupta et al. [42] report an average survival less than 3 months in patients with lung cancer whose θ p values are between 2 and 3°. This finding is confirmed in other studies [8, 13,  In this study, θ p < θ r may be explained because in the majority of cancer patients prevails that (Xc/H) p < (Xc/H) r for all value of (R/H) p , for each age group, gender and experimental group. This may suggest that component (Xc/H) p of the ordered pair ((R/H) p , (Xc/H) p ) prevails in the body bioelectric state of the cancer patient. From the biophysical point of view, (Xc/H) p cannot decrease until zero because θ p tends toward zero, which means the death of the cancer patients. This occur for all values of (R/H) p , confirming that (R/H) p and θ p are not linearly correlated. This may suggest that (Xc/H) p decreases until its minimum value that brings about a quicker decrease of (R/H) p than (Xc/H) p so that θ p stays in its normal range. From the thermodynamic point of view, a quick decrease of (R/H) p may mean the occurrence of different self-organized biophysical-chemical processes in the organism that reduce the body bioenergetics losses to guarantee its maximum survival and selfpreservation as a living system. One of the main characteristics of the biological organisms, as open systems, is to tend toward its maximum survival.
If net inductive effects of the patient [45] are neglected, Xc p may be due to the different total capacitive electrical reactance contributions of the tumor (named Xc t ), healthy tissue (Xc o ) and tumorsurrounding healthy tissue interface (named Xc o-t ). This interface is the tumor micro-environment and constitutes by the mixture of cancer cells, normal cells and other components. Xc p may be expressed in a first approximation as Xc p ≅ Xc t + Xc ο + Xc ο − t . As the capacitive contribution predominates in each tissue, Xc = 1/(2πfC), where C is the electrical capacity of the biological tissue under consideration and f is the working frequency (50 kHz). For this reason, Xc p , Xc t , Xc o and Xc o-t may be substituted for their respective electrical capacities. Therefore, C p may be calculated approximately as C p ≅ C t C ο C ο − t /(C ο C ο − t + C t C ο − t + C t C ο ), where C p , C t , C o and C o-t are the total electrical capacities of the entire patient, the tumor, the healthy tissue and the surrounding healthy tissue-tumor interface, respectively.
It has been experimentally documented that C ο > > C t (e.g., C t = 3.546 ± 1.931 pF for lung cancer and C o = 893 ± 572 pF for normal lung tissue) and the increase in capacitance of normal cells when cancer cells are added Fig. 2 Set of reference rectangles from 2 to 80 years of age (means ±2 standard deviations [16] were displayed in Table 1  to them [46]. The first finding is confirmed by other authors [47][48][49][50]. The second finding may mimic the mixture of cancer and normal cells in the tumor surrounding healthy tissue interface. These two findings may suggest that C ο C ο − t > > C t C ο > C t C ο − t and therefore C p ≅ C t . In addition, C p ≅ C t may also be obtained if contributions of Xc o-t to Xc p (C o-t to C p ) are neglected with respect to those of Xc t and Xc o (C t and C o ). In this case, (Xc) p ≅ (Xc) t + (Xc) ο and C p ≅ C t C ο /(C t + C ο ). C p ≅ C t is due to the fact as normal cells have dielectric constants and membrane electrical capacities greater than those of cancer cells [46][47][48][49][50]. In contrast, Frike and Morse [51] report that C t > C o . The fundamental contributions of Xc t and Xc o to Xc p may be argued because electrical activities occur mainly in the tumor and in healthy tissue (distant from the tumor). This result is expected because normal and cancerous cells are mainly confined in these two tissues and not in the tumor-surrounding healthy tissue interface. This aspect may explain in part why there are differences in the electrical properties of the tumor and healthy tissue, in agreement with others studies [47,48,52,53].
The finding C p ≅ C t confirms that electrical properties and biological characteristics of the cancer histological variety significantly influence the body homeostasis, quality of life, survival and body bioelectrical and physiological parameters of a patient with Ts/Tns. Nevertheless, the tumor-surrounding healthy tissue interface cannot be completely neglected because it influences the electrical-chemical microenvironment of the tumor, which is related to the aggressiveness and metastasis of it [54][55][56] and its protection against the attack of cellular and humoral elements of the immune system [57,58].
Due to the close relationship between the electrical and physiological parameters of a biological tissue [8,21,22,34,44,58,59], changes of C t and Xc t may be related to those of the transmembrane potential and the membrane permeability of cancer cells [47]. In turn, these changes have been related to other alterations observed in cancer cells as high mitotic activity, increase in the electronegativity of the extracellular surface, changes in intracellular and extracellular ionic concentrations, breakdown of homeostasis of electronic transport in the cell membrane, the depletion of adenosine triphosphate, the failure of the contact inhibition mechanism, morphological changes, aggressiveness, metastatic capacity, among others [47,56,59]. This leads to an alteration of the body homeostasis in cancer patients.
Loss of body homeostasis of a patient (child or adult) with cancer may lead to the decrease of the body cell mass and changes of its cell metabolism, body composition and distribution of total body water, as reported in [33,60,61]. This may explain the cachexia and modifications in (R/H) p , (Xc/H) p and θ p observed in cancer patients, explaining in part why (Xc/H) p < (Xc/H) r for all (R/H) p value observed in the majority of patients with Ts/ Tns. This finding may suggest that losses of the body energy reserves of the patient with Ts/Tns (related to (Xc/ H) p ) are faster than the body heat loss (related to (R/H) p ). This corroborates that θ p < θ r , as in [15,43], and the body metabolism and body composition alterations of these cancer patients, as in [34,60,61]. It is important to note that heat loss is noticeable as R p increases.
The finding (R/H) p > (R/H) r observed in some patients with Ts/Tns may be explained because body heat loss and water and ions body imbalances in cancer patient are noticeable compared to those in apparently healthy subjects. In turn, these alterations may be related to the biophysical-chemical-bioelectrical-energetic changes in the cell membrane, as discussed above.
Despite the increase of (R/H) p in these cancer patients, θ p decreases slightly with respect to θ r , for each gender and age group. This may suggest that these patients still have sufficient energy reserves to compensate the possible heat loss. If these losses prevail, θ p would decrease and therefore a low quality of life and short survival of the patient with Ts/Tns, in agreement with [42]. This statement would be noticeable when Xc p also decreases, in accordance with [37].
In general, |Z| and θ fix the position of any bioelectrical state in the plane R-Xc (R/H-Xc/H). The Cuban experience suggests that the simultaneous analysis of these two bioelectrical parameters is a necessary but not sufficient condition to absolutely differentiate one cancer patient from another or patients with any pathology from a population of apparently healthy subjects [30,62]. This statement may be argued because cancer patients and apparently healthy individuals are inside 50 and 75% tolerance ellipses. Some of these patients evolve unfavorably during and after the application of chemotherapy. Nevertheless, other cancer patients out 95% tolerance ellipse (3.5 < θ p ≤ 5) evolve favorably after this therapy [62]. Additionally, similar results are observed in lung cancer adult patients treated with surgery, radiotherapy, chemotherapy and/or immunotherapy (unpublished data). On the other hand, patients with acquired immune deficiency syndrome are also inside 50, 75 and 90% tolerance ellipses. In addition, there are no significant differences in body bioelectric parameters and body composition of these patients compared with those of an apparently healthy individual population [63].
The above-mentioned is why d pr is proposed in this study. d pr does not contradict |Z| or θ, but they complement each other. It should be expected that there is a minimum value of d pr , from which a sick subject can be differentiated from an apparently healthy subject, although the patient is in any position inside the ellipse 95% tolerance ellipse. Other distance criteria for d pr may be used, such as Mahalanobis distance [22] or a Hausdorff distance [64]. For instance, Hausdorff distance may be suggested to calculate this minimum distance. A longitudinal study is required to know this minimum value of d pr .
From bivariate statistics, it is well known that tolerance ellipses can be applied if R and Xc (R/H and Xc/H) are strongly correlated (r ≥ 0.8) [23,31]. The values of r show in Table 1 are less than 0.8 (correlation strength between moderate and fair). Rigorously speaking, the tolerance region should not be an ellipse but a rectangle in this R-Xc (R/H-Xc/H) plot, in agreement with [23]. This and the low correlations of r * (fair-poor) among R p , Xc p and θ p ((R/H) p , (Xc/H) p and θ p ) are why the rectangular tolerance region is used in this study. Rectangular tolerance region has been used in other studies [65][66][67]. In some studies, elliptical and rectangular tolerance regions are simultaneously analyzed [68,69]. Additionally, the representation of multiple rectangles in the same XY plane is possible taking into account the work published by Wald [70].
As ellipse tolerance and rectangular tolerance regions (95%) are the same and the rectangle and the ellipse are both centered at the same point, an ellipse can be inscribed in a rectangle [24]. In this case, it is easy to demonstrate that the ratio of the area of the rectangle/ area of the ellipse is 1.27 and the difference between their respective areas is 0.21L 1 L 2 . As a result, both areas are approximately equal. L 1 (L 1 = 2a 1 ) and L 2 (L 2 = 2a 2 ) are the length and width of the rectangle, respectively. The variable a 1 is the length of the semi-major axis of the ellipse whereas a 2 is the length of the semi-minor axis. L 1 = 2a 1 and L 2 = 2a 2 guarantee the biggest area of an ellipse within a rectangle.
Unlike the studies reported in this area of knowledge, the body bioelectric state of each cancer patient is individually represented in this study. This will permit an individualized integral diagnosis and the proposal of a personalized therapy for each of them. In addition, the analysis on animals or humans is suggested individually in a previous study [71]. Therefore, each cancer patient is represented in the Xc/H versus R/H plot, according to its age and gender.
Our results suggest performing a longitudinal study that permits to know how bioelectrical and physiological parameters of these patients with Ts/Tns change over time and what relationships can be established between them. This longitudinal study will allow to establish prognostic indicators of the possible evolution of a cancer patient under the application of a therapeutic scheme.

Conclusions
In conclusion, the BIA is feasible to individualize body bioelectrical parameters and body bioelectric state in newly diagnosed cancer children and how differ from those in apparently healthy subjects, for the same age group and gender. Additionally, the tumor electrical properties may have a noticeable role in changes of body bioelectric-physiological parameters of these newly diagnosed cancer children.

Availability of data and materials
Original data appear explicitly in Table 2. Nevertheless, any information and additional data available on request to the corresponding author (berguesc@yahoo.com).

Ethics approval and consent to participate
The final protocol was approved by the Ethics committee (Current Controlled trials BIACANCER12032009, 12 March 2009) and Scientific Board of the hospital Infantil Sur Antonio María Béguez César, Santiago de Cuba. Written Informed Consent is obtained from each participant before entering the trial.

Consent for publication
Not applicable.

Competing interests
The authors declare that they have no competing interests. Author Victoriano Gustavo Sierra González was employed by the company Grupo de las Industrias Biotecnológica y Farmacéuticas (BioCubaFarma, La Habana, Cuba). The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.