Fig. 8

Cholesterol depletion enhances the levels of IL-10 in the cultured media. IL-10 levels in conditioned media were measured by ELISA revealing significantly higher levels of IL-10 in conditioned media collected from 2 to 24 h after MbCD and Wnt5a treatment, when compared to untreated cultures (0 h). All samples were analyzed in triplicate and results of cytokine concentration were expressed in pg/mL and were mean and standard error of three different experiments. *p < 0.05 and ***p < 0.001; ANOVA versus control group (0 h)