Fig. 1

Representative Western blot. Proteins (40 μg) from two human bronchial epithelial cell lines (16HBE14o- and CFB41o-), primary human nasal epithelial (HNE) cells and human umbilical vein endothelial cells (HUVEC) were isolated by using 2 % Triton X-100 and separated on a 10 % SDS-PAGE. To identify the characteristic protein bands of vimentin (a) and keratin (b) we used a mouse monoclonal anti-vimentin and a mouse monoclonal anti-keratin antibody (Dianova), respectively. As secondary antibody we used a goat anti-mouse IgG-HRP conjugated antibody (Dianova). We detected a specific band of vimentin in the range of 57–60 kDa solely in the HUVEC sample and a specific band of keratin in the range of 45 kDa in all separated samples