Fig. 3
From: Optimization of CFTR-mRNA transfection in human nasal epithelial cells
Time course of a typical transepithelial measurement. Shown is the short circuit current (Isc) in HNE cells (non-transfected = controls) (a) and wtCFTR-mRNA transfected HNE cells (b) respectively. After the application of the cAMP-cocktail Isc smoothly increased in the control cells as shown in (a). In a second step, the chloride current was inhibited with the specific inhibitor CFTRinh172. When HNE cells were transfected with wtCFTR-mRNA, Isc increased, by the application of cAMP, which could be inhibited by the inhibitor CFTRinh172. The cAMP activation of CFTR is boosted by the transfection demonstrating functional expression of CFTR