Fig. 6
From: Optimization of CFTR-mRNA transfection in human nasal epithelial cells
Statistical evaluation of the wtCFTR-mRNA time dependency. Shown are the short-circuit values of CFTR activation by cAMP addition and the inhibition by CFTRinh172 application in HNE cells. a The cAMP activated chloride secretion is decreased according to time periods. Twenty-four hours after mRNA transfection with the optimal dose of 0.6 μg/cm2 the highest CFTR expression (9.3 ± 2.0 μA/ cm2; n = 19) was achieved. In comparison, longer time periods produced a decrease in the CFTR current. Nevertheless, we confirmed a positive CFTR activation over a period of 72 h (5.2 ± 0.6 μA/ cm2; n = 6). b The inhibition by the CFTRinh172 is expanded in the wtCFTR-mRNA transfected cells. Since we could not observe a reduction by the inhibitor in the control cells, we found a 72.93 % inhibition of the cAMP activated CFTR current in wtCFTR-mRNA transfected cells after 24 h (n = 19). Therefore, the CFTR inhibition was found 48 h (37.18 %) and even 72 h after transfection (27.08 %) (*p < 0.05; **p < 0.01)