Fig. 7
From: Optimization of CFTR-mRNA transfection in human nasal epithelial cells
Western blots analyses. Total membrane proteins (40 μg) from HNE cells were isolated using 2 % Triton X-100 and separated in a 7.5 % SDS-PAGE. To identify CFTR, we used a monoclonal anti-CFTR antibody that detects a CFTR band in the range of 170 kDa. In addition, we detected tubulin in the range of 55 kDa. a HNE cells were transfected with wtCFTR-mRNA (0.6 μg/cm2) at different time periods (24 h, 48 h). b Statistical evaluation of the relative CFTR protein amount in transfected cells. Twenty-four hours after wtCFTR-mRNA transfection the amount of CFTR protein was increased (1.5-fold) compared to controls