Analytical validation of an mRNA-based urine test to predict the presence of high-grade prostate cancer

Table 3 The analytical measurement range of the assays for DLX1, HOXC6 and KLK3

Target	LOD in copies/RT-PCR (Avg Cp)	LLOQ in copies/RT-PCR (Avg Cp)	ULOQ in copies/RT-PCR (Avg Cp)
DLX1	10 (37.24)	20 (36.00)	10⁷ (16.74)
HOXC6	10 (38.26)	20 (36.01)	10⁷ (16.40)
KLK3	10 (35.97)	20 (35.20)	10⁷ (16.74)

Avg average, Cp crossing point, DLX1 Distal-less Homeobox 1, HOXC6 Homeobox C6, KLK3 Kallikrein-3, LOD Limit of Detection, LLOQ Lower Limit of Quantification, RT-PCR reverse-transcriptase polymerase chain reaction, ULOQ Upper Limit of Quantification

ISSN: 2396-832X