Fig. 5

Changes in serum free aceneuramic acid concentrations measurement (Trial-2). In Trial-2, serum free aceneuramic acid not bound to protein or lipid was measured, focusing on concentration increase due to investigational drug administration. Tough physiologically existing free aceneuramic acid is also measured, the concentration after administration clearly increased in each case. a. Single administration for each individual subject. b. Single administration on average. Mean changes ± standard deviation, n = 3 for each group. c. Three-times-per-day administration for each individual subject. In three-times-per-day administration, the 2nd and 3rd administration is 8 and 14 h after the 1st administration. d. Three-times-per-day administration on average. Mean changes ± standard deviation, n = 3 for each group. e. Three times per day for 7-day administration for each individual subject. f. Three times per day for 7-day administration on average. Mean changes ± standard deviation, n = 3 for each group. In all cases of 7-day administration though individual variations were large, a clear increase in concentration was observed during administration and was maintained for 7 days. The peak of the trough value was different in each subject but was confirmed within 7 days