Fig. 1
From: Notch pathway connections in primary leukaemia samples of limited size
Validation of primer design strategy. a Efficiency: PCR reactions amplifying cDNA fragments with F1 and F2 semi-nested primers should have the same efficiency. The efficiency of the designed primers was tested on the mixture of all cell line cDNA samples. b Competition: 12 primers present in the same reaction should not interfere with each other. After the first multiplex PCR reaction using the cDNA mixture as a template, separate PCR reactions were set on with semi-nested F2 primers to analyse the specificity of the reaction. M (100 bp DNA Ladder), all amplicons cited with the name of the gene; longer amplicons produced by F1/R and shorter by F2/R primers