Fig. 6

The expression of adenosine receptors in T cells and inhibition of proinflammatory cytokine release and viral production by adenosine. RT-PCR results are shown for relative expression of A1R, A2AR, and A2BR in CD4⁺ (a) and CD8⁺ (b) T cells isolated from HCs (n = 5) and TPs (n = 7). c Immunofluorescence diagrams show that A2AR is constitutively expressed on CD4⁺ and CD8⁺ T cells. d MFI values of A2AR expressed on CD4⁺ and CD8⁺ T cells were higher in patients with AIDS. e PBMCs from TPs were stimulated with anti-CD3 and anti-CD28 antibodies. Virus titers were determined following 24 h of culture in the presence or absence of CADO. f MFI of the HIV-p24⁺ CD4⁺ T cells in gated HIV-p24⁺ CD4⁺ T cells in response to CADO under anti-CD3 and anti-CD28 stimulation. g Pooled data showing the TNF-α expressing CD4⁺ T cells and CD8⁺ T cells from TPs in response to various concentration of CADO under stimulation with anti-CD3 and anti-CD28 antibodies. *p < 0.05, **p < 0.01, ***p < 0.001