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Fig. 2 | Translational Medicine Communications

Fig. 2

From: N-acetyl cysteine prevents pain and hypersensitivity of bleaching agents without affecting their aesthetic appeal; evidence from in vitro to animal studies and to human clinical trials

Fig. 2

NAC protects the gingival cells from undergoing cell death in the presence of bleaching agents in rats. 8-week old male rats were divided into three groups; No bleaching agent- control (left panel) (n = 3), bleaching agent only (middle panel) (n = 3) and bleaching agent with NAC (right panel) (n = 3). Four hours after application, the palatal tissues were extensively washed with sterilized saline, and the tissues were removed surgically and treated with 0.25% trypsin-EDTA and 0.1% collagenase to prepare single cell suspensions as described in the Materials and Methods section. The surface topography was analyzed using Scanning Electron Microscopy, magnification 500X and bar = 100 μm for the upper panels and, magnification 2000X and bar = 20 μm for the lower panels (A)

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