AMP5A modulates Toll-like receptors 7 and 8 single-stranded RNA immune responses in PMA-differentiated THP-1 and PBMC

Table 2 Multiple cytokines and chemokines are significantly suppressed by AMP5A in CL075-stimulated, PMA-differentiated THP-1 cultures. PMA-differentiated THP-1 were exposed to saline or AMP5A and then stimulated with 5 µg/ml CL075 for 24 hours. Cytokine/chemokine release was determined by 48-multi-plex array, and data are presented as fold change ± STD or percent inhibition (n = 3, p-value = ≤ 0.05 for fold change versus theoretical fold change of 1)

Cytokine/Chemokine	Gene ID	fold change	STD	% Inhibition	p value
CXCL10	CXCL10	0.03	0.02	97%	0.00010
IL-6	IL6	0.08	0.09	92%	0.00287
IL-12p40	IL12B	0.09	0.01	91%	0.00004
MCP-1	CCL2	0.10	0.01	90%	0.00007
MCP-3	CCL7	0.14	0.05	86%	0.00110
IL-1β	IL1B	0.30	0.10	70%	0.00686
MIP-1β	CCL4	0.32	0.05	68%	0.00181
MIP-1α	CCL3	0.33	0.07	67%	0.00352
IL-12p70	IL12A	0.37	0.07	63%	0.00384
MIG/CXCL9	CXCL9	0.43	0.11	57%	0.01174
IL-1RA	IL1RN	0.43	0.22	57%	0.04451
TNFα	TNF	0.44	0.05	56%	0.00308
RANTES	CCL5	0.47	0.01	53%	0.00836
IL-27	IL27	0.47	0.03	53%	0.00100
GM-CSF	CSF2	0.48	0.10	52%	0.01103
G-CSF	CSF3	0.52	0.15	48%	0.02971
IL-15	IL15	0.59	0.05	41%	0.00443
Fractalkine	CX3CL1	0.65	0.12	35%	0.03597
IL-17F	IL17F	0.67	0.10	33%	0.02683
IL-18	IL18	0.67	0.07	33%	0.01688
IL-5	IL5	0.68	0.08	32%	0.02198
GROα	CXCL1	0.70	0.05	30%	0.00809
TGFα	TGFA	0.72	0.04	28%	0.00647
MDC	CCL22	0.80	0.04	20%	0.01443
IL-2	IL2	0.80	0.08	20%	0.04981
EGF	EGF	0.81	0.07	19%	0.04440

ISSN: 2396-832X