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Fig. 4 | Translational Medicine Communications

Fig. 4

From: Targeted quantitation of CFTR protein expression in vivo using immunoprecipitation & parallel reaction monitoring tandem mass spectrometry

Fig. 4

Comparison of peak intensities of four hCFTR peptides (AVQPLLLGR, STLLSAFLR, GQLLAVAGSTGAGK and LSLVPDSEQGEAILPR) generated by on-beads digestion vs off-beads digestion. hCFTR protein (5 ng) spiked in mouse lung membrane protein extracts (50 mg lung tissue) was used as test sample. Streptavidin magnetic beads and biotinylated antibody were used for IP. Data were processed by skyline software

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Translational Medicine Communications

ISSN: 2396-832X

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