Fig. 4

Changes in peripheral blood leukocyte subsets in male and female ALM-treated and Saline control animals after ACLR surgery. A Average circulating lymphocytes, monocytes and neutrophils counts at baseline, prior to surgery (-24 h), and at 1, 24, 72 and 120 h after ACLR surgery. B Relative cell-surface expression of CD11b/c and CD62L on circulating neutrophils at 1 h postoperative. C Relative cell-surface expression of CD11b/c on peripheral blood monocytes to 120 h postoperative. D Total number of circulating non-classical (SSC^Lo CD43^HI) monocytes at 120 h postoperative. Changes in the number of circulating E precursor T regulatory cells (T_regs; CD4⁺CD25⁻FoxP3⁺), and F conventional T_regs (CD4⁺CD25⁺FoxP3⁺) to 120 h postoperative. Data show mean ± SEM. Mixed ANOVA, Tukey post-hoc test. * p < 0.05, male ALM, compared to female ALM. ⁑ p < 0.05 male Saline, compared to female Saline. ^ p < 0.05 male ALM, compared to male Saline. # p < 0.05 female ALM, compared to female Saline. Shaded area in C, E and F shows mean ± SEM for healthy baseline male (blue) and female (red) animals (n = 8 per sex). MFI, median fluorescence intensity; Tx, treatment