Fig. 6

SPT-2101 prevents LPS-induced alterations of cochlear immune and inflammatory response transcripts. (A) Heatmap representation of PCR array expression data (2^–ΔCt) from control or LPS-injected rats, 4, 25 and 72 h after injection. At least 3 cochleae from different rats per group were studied. (B) RT-qPCR expression levels of candidate genes selected from A in control (white), LPS (red) or LPS plus SPT (green) experimental groups, 72 h after the first LPS injection. Results were calculated as 2^–ΔΔCt (RQ, expressed as mean ± SE), using Tbp as the reference gene and normalized to control rats without LPS. At least 3 rats were included for each group. Statistical significance was analyzed by either one-way ANOVA or the Kruskal-Wallis test (*p < 0.05; **p < 0.01; ***p < 0.001 compared to controls; ##p < 0.01, ###p < 0.001 compared to LPS).