Fig. 3
From: Efficacy of derinat as a treatment for murine and androgenetic alopecia (AGA) patients
Treatment with Derinat affects HF cycle progression due to a decrease in intracellular ROS production. A Effect of Derinat on skin ROS production. The intracellular ROS production in dorsal skin and HFs was analyzed by DHE staining. B Quantification of intracellular ROS production from (A) (mean ± SD; *, p < 0.05). C Effect of Derinat on TRPCs activity in skin cells. After the application of ATP (small black bars) to activate TRPCs via the PLC pathway in Ca2+-free balanced salt solution, extracellular CaCl2 was added (large black bar) in keratinocytes (KC), fibroblasts (FB), and dermal papilla cells (DP). The Ca.2+ signals represent the mean value of 30 cells. D Effect of Derinat on intracellular ROS production in skin cells. The intracellular ROS production level with an average fluorescence intensity of more than 100 skin cells from (D) is quantified in (E) (mean ± SD; *, p < 0.05; **, p < 0.01)