Fig. 3

IRE1 downstream pathways have opposing roles in prognosis and presence of immune cells in TME. A Kaplan Meier survival analysis based on the expression of XBP1 and MAPK8 was performed dividing patients into 4 groups. Only High-Low compared to Low–High was significantly different (p = 0.03; High-High vs. Low–High: p = 0.17; Low-Low vs. Low–High: p = 0.25; High-High vs. High-Low: p = 0.30; Low-Low vs. High-Low: p = 0.46; High-High vs. Low-Low: p = 0.92). Expression of immune cell markers (B) and immune cell meta-signatures (C) in Low–High and High-Low groups. Mann–Whitney U test was carried out comparing microarray expression data from the TCGA cohort. D Feature plot depicting XBP1/MAPK8 expression ratio in single cells of the 9 glioblastoma samples from scRNA-seq data. E Comparison between the ratio of XBP1 and MAPK8 average expression only in tumor cells with the percent of immune cells in each sample. Each dot represents one tumor sample from scRNA-seq data. Samples’ IDs are indicated. Pearson's correlation coefficient (r) and p-value (p) are shown