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Prognostic value of morphologic and morphometric analyses in IgA nephropathy biopsies
Translational Medicine Communicationsvolume 1, Article number: 7 (2016)
IgA nephropathy (IgAN) exhibits a variable course ranging from a benign condition to progressive renal failure with a high proportion of patients undergoing end-stage renal disease in the long term. It is unclear how to predict the risk of this progression. Mesangial C4d deposition has been found to be associated with a poor prognosis. Our aim was to search histological lesions with possible prognostic value in IgAN biopsies performed at the time of the diagnosis.
Clinical and laboratory records of 44 patients undergoing renal biopsy were reviewed. IgAN was diagnosed in 32 patients and minimal change disease (MCD) in 12.
C4d deposition, glomerular endothelial cell area, glomerular and interstitial macrophages were evaluated in all biopsies. Clinical and laboratory data were available in all IgAN patients at the time of diagnosis and in 21 patients at the end of follow-up (mean follow-up 48.09 ± 19.69 months).
All IgAN and MCD biopsies were C4d negative. The number of glomerular macrophages and the area of glomerular endothelial cells in IgAN were greater than in MCD (P < 0.001 and P < 0.0001, respectively), indicating glomerular inflammation associated with endothelial cell enlargement. Glomerular macrophages positively correlated with diffuse IgA and fibrinogen immunoreactivity (P < 0.02 and P < 0.002, respectively).
Interstitial macrophages positively correlated with segmental glomerulosclerosis (P < 0.01) and tubular atrophy (P < 0.001), according to the Oxford classification. They correlated with hypertension (P < 0.03) and renal functional impairment both at the time of biopsy and at the end of follow-up. The receiver operating characteristic analysis curve and a cut-off value of ≥19.5 macrophages per high power field showed 75.0% sensitivity and 88.9% specificity in predicting worse clinical outcome. A Poisson generalized regression fit model showed that hypertension and tubular atrophy were the most significant factors in predicting a high number of interstitial macrophages.
The number of interstitial macrophages correlated with hypertension, histological damage, functional impairment at the time of biopsy and worse outcome. A high number of interstitial macrophages in C4d negative biopsies may allow the identification of patients at increased risk of progression to renal failure.
IgA nephropathy (IgAN) has an incidence of approximately 2.5/100000/year in adults . It is characterized by the presence of glomerular IgA deposition in patients with high levels of immune complexes formed by aberrantly glycosylated IgA and endogenous antiglycan antibodies. IgA deposition is predominant, and IgG, IgM and C3 deposition are frequently observed.
IgA-containing immune complexes deposited in the glomeruli are associated with inflammation, proliferation of mesangial cells, and synthesis of extracellular matrix molecules . The clinical course and the histopathological presentation of IgAN are highly variable [2–4]. IgAN may exist sub-clinically . Although it usually initiates as a benign condition, in 15–20 years 15–40% of the patients progress to end-stage renal disease (ESRD) [3–5]. Hypertension, some renal function parameters and histopathological features are known prognostic risk factors. These parameters, however, may only indicate the extent of the disease at a particular time, such as the stage of the disease at the time of diagnosis . For instance, tubule-interstitial damage, associated with a rapid development of ESRD [2–4], indicates an advanced disease. The majority of patients present haematuria with or without proteinuria, and a slowly progressive course. A high percentage of these patients, however, will experience a decline in renal function with a rate of progression varying among patients [3, 4]. Thus, it would be important and also challenging to predict the risk of progression.
In renal transplant patients the C4d fragment of the C4 complement component is released during activation of the classic complement pathway; its deposition on the surface of endothelial cells of peritubular and glomerular capillaries is a marker of antibody-mediated acute rejection, and poor graft survival . In IgA nephropathy complement activation typically occurs via the alternative pathway, thus without C4d deposition . However, C4d deposition has been reported in approximately 25% of IgAN cases,  probably due to activation of the mannose-binding lectin pathway . As in patients with antibody-mediated renal acute rejection, this is predictive of a poor prognosis . C4d deposition in antibody-mediated acute rejection biopsies is associated with a high number of interstitial macrophages and glomerulitis [9, 10]. Acute transplant glomerulitis, defined by the presence of mononuclear cells-predominantly macrophages-in the glomeruli and endothelial cell enlargement  in some cases progresses to chronic glomerulopathy, characterized by capillary basement membrane multilayering, proteinuria and decline of renal function [10, 11].
In IgAN the mechanism of haematuria is unknown, endothelial cell damage and alterations of the glomerular basal membrane may contribute to its development [4, 12]. Whether endothelial cell enlargement is present in IgAN glomeruli has not been investigated. The aim of this study was to evaluate the prognostic value of histological lesions such as glomerulitis and interstitial macrophages in IgAN biopsies. The associations between histological lesions and proteinuria, creatininemia, glomerular filtration rate and hypertension were evaluated, and only patients with C4d negative renal biopsies were included in the study. The patients showed a classical clinical presentation with haematuria and moderate proteinuria. Minimal change disease (MCD) biopsies were studied for comparison, considering that in this disease glomerular changes at light microscopy and activation of the complement system are not detected .
Clinical and laboratory records of 44 patients undergoing renal biopsy consecutively between January 2003 and December 2012 at our Institution were reviewed. IgAN was diagnosed in 32 patients (21 males and 11 females, mean age 35.9 ± 11.69 years) and MCD in 12 (6 males and 6 females, mean age 39.50 ± 10.81 years). Patients included in the study were more than 15 years of age and underwent biopsy before any immune-suppressive treatment. All IgAN patients showed C4d negative renal biopsies. Patients with diabetes mellitus, autoimmune diseases, abnormal hypergammaglobulinemia, and liver diseases were excluded from the study. After biopsy, 7 patients were treated with renin-angiotensin system blockers (RASB), 8 with corticosteroids, and 15 with RASB and corticosteroids. Two patients with normal renal function, proteinuria < 1 g/24 h and biopsies showing only mild mesangial hypercellularity received no treatment. Haematuria, proteinuria (g/24 h), serum creatinine level, (reference range obtained by our clinical laboratory: males = 0.66–1.25 mg/dl, females = 0.52–1.04 mg/dl), blood pressure, and estimated glomerular filtration rate (eGFR) (ml/min per 1.73 m2) were recorded at the time of biopsy. Hypertension was defined as systolic blood pressure > 135 mmHg and/or diastolic blood pressure > 85 mmHg or the use of anti-hypertensive agents. In all hypertensive patients typical vascular lesions were observed in the biopsies. Evaluation of the clinical outcome was assessed using the Kidney Disease Outcome Quality Initiative (KDOQI) guidelines , which define 5 stages based on eGFR evaluation (stage 1: eGFR ≥ 90 ml/min; 2: eGFR = 60–89 ml/min; 3: eGFR = 30–59 ml/min; 4: eGFR = 15–30 ml/min; 5: eGFR = <15 ml/min). Follow-up time was considered as the interval time between renal biopsy and the last outpatient visit, haematuria, proteinuria, serum creatinine levels and eGFR data were available for 21 patients (mean follow-up: 48.09 ± 19.69 months). They were treated with methylprednisolone (0.5–1 mg/kg/die) (n = 3), RASB (n = 7) or methylprednisolone (1.5–1 mg/kg/die) in combination with RASB (n = 9). Two patients received no treatment. To evaluate the outcome the decline of the eGFR was considered.
Histopathology and immunohistochemistry
Formalin-fixed paraffin-embedded sections were stained with hematoxylin-eosin, PAS and Masson trichrome. Direct immune-fluorescence was performed on fresh frozen tissue with FITC-conjugated polyclonal antibodies to detect IgG, IgM, IgA, C3 and fibrinogen (Dako, Glostrup, Denmark). IgAN biopsies were scored according to the Oxford  and the 3-Grade classification . According to the Oxford classification  each glomerulus was scored for mesangial hypercellularity (M0-M1, M1 indicated hypercellularity in more than 50% of glomeruli). The other parameters were endocapillary hypercellularity (present/absent: E0-E1); segmental glomerulosclerosis (present/absent: S0-S1) and tubular atrophy (T0 = < 25%, T1 = 26–50% and T2 = > 50% of the cortical area). According to the 3-Grade classification  biopsies were scored as G1 when a mild increase in mesangial cells and no glomerulosclerosis or tubulo-interstitial changes were detected, as G2 when at least one of the following features was detected: moderate increase in mesangial cellularity (focal or diffuse), endocapillary proliferation, cellular crescent, (up to 50% of glomeruli), focal segmental glomerulosclerosis, tubular atrophy and interstitial fibrosis (up to 1/3 of the cortical area), as G3, when cellular crescents were present in more than 50% of glomeruli, and/or fibrous crescents or global glomerulosclerosis were present in more than 1/3 of glomeruli, and/or segmental glomerulosclerosis involved more than 50% of glomeruli and/or tubular atrophy/interstitial fibrosis involved more than 1/3 of the cortical area.
Sections were stained for immunohistochemistry using the Bond Polymer Refine Detection method (Leica Biosystem, Wetzlar, Germany) after high pH (pH 9.0) buffered pre-treatment (Leica Biosystem), using the murine monoclonal antibody (MoAb) anti-CD68 (Dako), recognizing macrophages. Quantification of glomerular macrophages was performed counting CD68+ cells in each glomerulus and dividing the sum obtained in each biopsy by the number of scored glomeruli (magnification 40). Interstitial macrophages in the cortical area were counted in 10 high power fields (HPF) and the mean number per HPF was reported (magnification × 40). For detection of C4d paraffin sections were stained using the XT Ultraview DAB v3 method (Ventana Medical System, Tucson, AZ, USA) after antigen retrieval by pressure-cooking using a polyclonal anti-C4d antibody (Biomedica, Vienna, Austria). Biopsies from 12 patients with MCD were used as negative controls, and two biopsies from patients with lupus glomerulonephritis as positive controls.
Staining of endothelial cells with anti-CD31 murine MoAb (Dako) was performed using the Ultravision LP polymer detection method (Thermo Fisher Scientific, Freemont, CA, USA) after antigen retrieval by heating the sections in Tris-EDTA buffer (pH 8.0). Primary antibodies were replaced by irrelevant matched MoAb or immune serum, as appropriate, as a control for non-specific staining.
Glomerular capillary loops were examined after CD31 immunostaining of endothelial cells as previously described  using a Zeiss Axioskop 40 light microscope (Carl Zeiss, Gottingen, Germany) equipped with a Coolsnap Videocamera (Photometrics, Tucson, AZ, USA). MetaMorph Software System (Universal Imaging Corp Molecular Device Corp, CA, USA) was used to analyse images. Only full cross-sectioned loops were measured. Capillary loops showing endothelial cell nuclei were not considered. Leukocytes or red cells within capillaries were considered as part of the lumen. The external and the internal capillary outlines were manually delineated at × 40 magnification. The total area of the capillary (TCA), and the area of the capillary lumen (LCA) were automatically measured. The percentage of the total area of the capillary occupied by endothelial cells (ECA%) was defined as the ratio of (TCA - LCA)/TCA × 100). Analyses were conducted on 44 biopsies (32 IgAN and 12 MCD), and 3 glomeruli were examined in each biopsy. The area of capillary loops was measured in IgAN glomeruli (1839 loops) and in MCD glomeruli (605 loops). Glomerular areas were evaluated as previously described .
Results are expressed as mean ± SD. The Pearson χ 2 test, Wilcoxon/Kruskal-Wallis test, Pearson’s correlation test, and one-way ANOVA were used for statistical analysis. For all analyses statistical significance was defined as P < 0.05. To estimate the performance as a prognostic marker of interstitial macrophages per HPF the receiver operating characteristic (ROC) analysis curve was constructed and the area under the ROC curve (AUC) and the corresponding 95% confidence interval (C.I. 95%) were evaluated. The most appropriate cut-off value of interstitial macrophages per HPF to predict clinical outcome was determined by the point of interstitial macrophage number with maximum sum of sensitivity and specificity. Poisson generalized regression fit model and stepwise regression analysis were used to select the explanatory variables for the standard least square full factorial regression model. Statistical analyses were performed by JMP 11 software (SAS Institute Inc. Cary, NC).
Glomerular macrophages and immunofluorescence pattern in IgAN and MCD biopsies
No significant differences in age and male/female ratio were present between IgAN and MCD patients. In IgAN biopsies the number of macrophages per glomerulus was significantly higher than in MCD (6.31 ± 3.51 versus 1.11 ± 0.73, P < 0.0001). CD68+ macrophages in IgAN and MCD glomeruli are shown in Fig. 1a and b. IgA, IgM, IgG, C3 and fibrinogen immune-fluorescence staining was negative in all MCD biopsies. As required for the diagnosis, all IgAN patients showed predominant IgA immune-reactivity in the mesangial area of glomeruli, with diffuse positivity in 15 biopsies. Immune-reactivities for IgG, IgM, C3 and fibrinogen were observed in 11, 22, 32 and 18 biopsies, respectively. Diffuse positivity was present in 3, 9, 11 and 6 biopsies, respectively. The number of glomerular macrophages was significantly higher in biopsies with diffuse IgA positivity (n = 15) than in biopsies with IgA focal positivity (n = 17) (7.82 ± 3.65 versus 4.97 ± 3.14, P < 0.02), and it was markedly higher in biopsies with diffuse fibrinogen positivity (n = 6) than in biopsies with negative/focal fibrinogen positivity (n = 26) (10.18 ± 2.97 versus 5.41 ± 3.19, P < 0.002).
No significant difference was observed in the presence of diffuse versus focal/negative IgG, IgM or focal C3 staining (data not shown).
This finding suggests a positive correlation between the number of glomerular macrophages and the diffuse distribution of IgA and fibrinogen.
Glomerular endothelial cell area in IgAN and MCD biopsies
The area of the capillary loop occupied by endothelial cells (ECA%), was significantly higher in IgAN than in MCD biopsies (76.81 ± 5.89 versus 61.83 ± 8.39, P < 0.0001) (Fig. 2a). Glomerular areas in IgAN and MCD biopsies were not significantly different (18698 ± 6553 and 16895 ± 6858 μ 2, respectively P = 0.427). Representative CD31+ glomerular endothelial cells in MCD and IgAN are shown in Fig. 2b and c.
C4d deposition in IgAN and MCD biopsies
Glomerular, mesangial or peritubular C4d staining was not observed in either MCD or in IgAN biopsies (Fig. 3b and c). In two biopsies of lupus glomerulonephritis, included as positive controls, the glomerular staining pattern was diffuse and segmental, and peritubular capillaries were negative. Fig. 3a shows glomerular C4d positivity in a biopsy of lupus glomerulonephritis (class IV).
Glomerular and interstitial macrophages and histological grading
The number of interstitial macrophages per HPF in the cortical area was significantly higher in IgAN than in MCD biopsies (18.29 ± 5.01 versus 11.25 ± 4.90, P < 0.0001). CD68+ macrophages in IgAN and MCD interstitial cortical area are shown in Fig. 1c and d.
According to the Oxford Classification , in IgAN biopsies scored for mesangial cellularity (M0/M1) no differences in the number of glomerular or interstitial macrophages were observed (Table 1). Endocapillary hypercellularity was scored as E0 in all biopsies. In biopsies scored for glomerulosclerosis as G1 and for tubular atrophy as T1, the number of interstitial, but not that of glomerular macrophages was significantly higher than in G0 and T0 biopsies (P < 0.01 and P < 0.001, respectively) (Table 1). Using the 3-Grade Classification , a low number of macrophages was found to be associated with the absence of glomerulosclerosis and tubule-interstitial changes (G1) (P < 0.005) (Table 1).
Glomerular and interstitial macrophages: clinical data and outcome
In 32 IgAN patients haematuria, proteinuria, serum creatinine levels, eGFR and blood pressure were examined at the time of biopsy. At the end of follow-up haematuria, proteinuria, serum creatinine levels and eGFR data were available for 21 patients (mean follow-up: 48.09 ± 19.69 months). At the time of biopsy microscopic haematuria was present in 30 patients, gross haematuria in one, and no haematuria in one (n = 32). At the end of follow-up (n = 21), 14 patients showed microscopic haematuria The mean level of proteinuria at the time of biopsy was: 1.82 ± 1.19 g/24 h (n = 32). Proteinuria levels at the time of biopsy and at the end of follow-up did not correlate with glomerular or interstitial macrophages, or with any of the other histological lesions studied. Interestingly, in the 21 patients with available follow-up data the mean level of proteinuria at the end of follow-up (0.69 ± 0.78 g/24 h) was significantly lower than that observed at the time of biopsy (1.61 ± 1.01 g/24 h, P < 0.002) indicating a clear improvement of this parameter.
The number of interstitial, but not that of glomerular macrophages was significantly higher in patients with increased serum creatinine levels both at the time of biopsy and at the end of follow-up (P < 0.007 and P < 0.03, respectively) (Table 2).
Moreover, a significant inverse correlation between the number of interstitial but not of glomerular macrophages and the eGFR was observed at the time of biopsy (r = −0.463, P < 0.008 and r = −0.99, P = 0.5 respectively) and at end of the follow-up (r = −0.474, P < 0.03 and r = −0.9, P = 0.3, respectively) (Fig. 4).
In patients without hypertension (n =15) the number of interstitial macrophages per HFP was significantly lower than in patients with hypertension (n = 17) (15.97 ± 4.92 versus 20.93 ± 3.72, P < 0.03), whereas there was no difference in the number of glomerular macrophages (5.80 ± 3.62 versus 6.88 ± 3.68, P = 0.40). Patients with hypertension were older than those without hypertension (42.26 ± 9.46 versus 29.70 ± 10.39 years, P < 0.001), and in their biopsies one or more features of hypertensive nephrosclerosis were present: arteriolar hyalinosis in 5 patients, arteriolar or arterial hyperplasia of smooth muscle cells in 9, arterial proliferative intimal thickening in 3, arterial intimal fibrosis in 3, and global glomerular sclerosis in 1.
Evaluation of the clinical outcome was based on the eGFR at time of biopsy and at the end of follow-up according to the stages established by the KDOQI guidelines for chronic kidney disease . The 21 patients with follow-up data were considered. Twelve patients (group 1) had a favourable outcome: 7 were in stage 1 at the time of the biopsy and at the end of follow-up, and 5 showed an improved stage at the end of follow-up (stages 2, 3, 3, 3 and 5 at the time of diagnosis; stages 1, 2, 1, 2 and 1 at the end of follow up, respectively). The remaining 9 patients (group 2) had an unfavourable outcome: 6 had no improvement (stages 2, 2, 3, 3, 3 and 3 at the time of the diagnosis and at the end of follow-up) and 3 showed worse stages, with ESRD in two patients (stages 1, 3 and 3 at the time of diagnosis; stages 2, 5 and 5, respectively, at the end of follow-up). In group 1 biopsies the number of interstitial macrophages per HPF was significantly lower than in group 2 (17.34 ± 5.00 versus 22.15 ± 3.32, P < 0.02).
ROC curve analysis showed that the number of interstitial macrophages predicted an unfavourable outcome. AUC was 0.80 (C.I. 95% = 0.6–1.0; P < 0.0021) and a cut-off value of ≥19.5 macrophages per HPF showed 75.0% sensitivity and 88.9% specificity in predicting a worse clinical outcome. Among 11 patients with ≥ 19.5 interstitial macrophages per HPF, 8 (72.7%) had an unfavourable outcome, while among 10 patients with < 19.5 interstitial macrophages, only 1 (10%) had an unfavourable outcome (Fisher’s exact test: P < 0.0075). Therefore, the number of interstitial macrophages per HPF resulted a useful parameter to evaluate the clinical outcome.
The number of macrophages was not significantly associated with different therapeutic regimens in either the favourable or unfavourable patient group (P = 0.48 and P = 0.23, respectively). To control for covariance between the factors associated with a high number of interstitial macrophages a Poisson generalized regression fit model was performed. The number of interstitial macrophages was considered as a function of 6 variables: grade of the 3-Grade classification (G), segmental glomerulosclerosis (S), tubular atrophy (T), hypertension, serum creatinine level and eGFR. These variables were chosen since univariate analyses showed that they were significantly correlated with interstitial macrophage number. The whole model test yielded a significant fit (−Log Likelihood = 9.75; χ 2 = 19.5; P < 0.0034) in predicting the number of interstitial macrophages. We then performed variable selection using stepwise regression analysis for the 6 variables used as explanatory effects in the Poisson generalized regression. The criterion to add or delete a variable in the model was based on F-statistics, with a critical P-value of 0.05. Hypertension and tubular atrophy were selected as explanatory variables for the standard least square full factorial regression model. The selected markers accurately validated the estimate of interstitial macrophage number (actual versus predicted number: r = 0.67, P < 0.0006). Interestingly, hypertension appeared significantly nested within tubular atrophy (P < 0.013) as its positive effect on interstitial macrophage number occurred only in the absence of tubular atrophy. As shown in Table 3 a significantly positive effect of hypertension on the number of interstitial macrophages occurred only when there was no tubular atrophy. Tubular atrophy produced significantly increased interstitial macrophages independent of the hypertensive status.
Identification of histological lesions with prognostic value at the time of diagnosis is important to identify IgAN patients with high risk of progression. We evaluated C4d deposition, the area of glomerular endothelial cells, glomerular and interstitial macrophages in IgAN biopsies. We showed that a high number of interstitial macrophages is associated with hypertension, unfavourable histological grading and deterioration the renal function in C4d negative biopsy patients.
In agreement with the evidence of a good prognosis in C4d negative patients  only two patients underwent ESRD. Numerous glomerular macrophages and endothelial cell enlargement characterized the glomerulitis. Endothelial cell enlargement is one of the signs predicting chronic glomerulopathy in antibody-mediated renal transplant acute rejection . The glomerular endothelial cell area in IgAN has not yet been studied. We found that it was greater than in MCD, and similar in its extent to the area measured in acute transplant glomerulitis . Endothelial cell enlargement may indicate cell damage and contribute to the development of haematuria, with negative prognostic implications [4, 12].
The number of macrophages in glomeruli was positively correlated with diffuse deposition of IgA and fibrinogen, a result, to the best of our knowledge, shown for the first time. This is in line with in vitro studies investigating the mechanisms underlying the recruitment of glomerular macrophages. Human mesangial cells cultured with polymeric IgA from IgAN patients produce RANTES and monocyte chemoattractant protein-1 (MCP-1), potent chemotactic agents for macrophages . Moreover, MCP-1 is produced by human endothelial cells cultured with fibrinogen at physiological concentrations .
Using two different histological classifications we found that interstitial macrophages were associated with glomerulosclerosis and tubular atrophy. In glomerular diseases, glomerular damage precedes the interstitial fibrosis , thus, as also suggested by in vitro studies , it is possible that macrophage-mediated mechanisms may link these events.
Interstitial macrophages were previously correlated with poor long-term prognosis, but not with impaired renal function at the time of biopsy . Other authors have reported a correlation with impaired renal function at the time of biopsy and long-term prognosis . In patients with C4d negative biopsies a high number of interstitial macrophages correlated with impairment of renal function at the time of biopsy and at the end of follow-up, whereas the number of glomerular macrophages did not have any functional or prognostic value. This is in line with the concept that macrophages in different localizations display different functions . For instance, in IgAN and in renal transplant rejection biopsies the receptor for the chemokine RANTES (CCRR5) was expressed by interstitial but not by glomerular macrophages .
A persistent high level of proteinuria is associated with severe histological lesions, including high scores of Oxford classification parameters [4, 23, 25, 26]. In our study proteinuria levels did not correlate with histological findings. This might be due to the improvement of proteinuria levels observed at the end of follow-up.
Hypertension is a well-established risk factor for IgAN progression [3, 4]. For the first time in IgAN we found a positive correlation between the number of interstitial macrophages and hypertension. The systemic renin-angiotensin-aldosterone system and the intrarenal renin-angiotensin system contribute to the development of hypertension . In renal vascular hypertension the presence of macrophages is attributed to the pro-inflammatory activity of angiotensin II (ATII) . In rat models of ATII-induced hypertensive kidney injury macrophages were found in the interstitium, associated with MCP-1 expression in tubular cells and peritubular interstitial cells . Macrophages in turn promote hypertension [30, 31]. Infusion with ATII in mice was paralleled by macrophage infiltration of the vessel walls, elastin breakdown and collagen deposition  whereas M-CSF-deficient mice lacking mature macrophages exhibited a decreased hypertensive response . We found that the most relevant predictive factors for a high number of interstitial macrophages were hypertension and tubular atrophy. The absence of hypertension showed a protective effect, and in the absence of tubular atrophy hypertension was predictive of an increased number of interstitial macrophages. Notably, tubular atrophy was predictive of a high number of interstitial macrophages independently of the blood pressure status. RASB reduced blood pressure, MCP-1 expression and macrophage infiltration in some experimental hypertension models . However, when biopsies of patients with hypertensive nephrosclerosis treated with RASB were compared with those of untreated patients the number of interstitial macrophages was not reduced . Our data suggest that the therapeutic regimens did not influence the prognostic value of the number of interstitial macrophages. In IgAN the decision to treat patients with RASB alone or to add corticosteroids depends on the presence of signs associated with poor prognosis. In the Kidney Disease Improving Global Outcome (KDIGO) practice guidelines the administration of glucocorticoids is suggested when proteinuria is higher than 1.5 g/day and the eGFR is more than 50 ml/min after 6 months of RASB therapy. The guidelines mention that histological risk factors could be a good reason to combine RASB with glucocorticoids, although no trials have been performed . Glucocorticoids reduce macrophage infiltration and tubulo-interstitial injury in murine lupus nephritis . Thus, early combined treatment with RASB and glucocorticoids may improve the long-term prognosis in patients with pronounced macrophage interstitial infiltration.
A limitation in our study is the small sample size, thus, our results need to be validated with a larger cohort of patients. In addition, it will be important to assess the prognostic value of the number of interstitial macrophages in other primary glomerulonephritides.
A high number of interstitial macrophages in C4d negative biopsies of IgAN patients predicts an unfavourable outcome. Our data encourage the identification of novel anti-fibrotic therapies targeting macrophages.
Area under the curve
Area of the capillary occupied by endothelial cells
Estimated glomerular filtration rate
End stage renal disease
High power field
Kidney Disease Outcome Quality Initiative
Area of the capillary lumen
Minimal Change Disease
Renin-angiotensin system blockers
Receiver operating characteristic
Total area of the capillary
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We thank Dr. Tiziana Musso for helpful discussion and Dr Chiara Cerletti for critical review of the manuscript.
Supported in part by funds from Ministero dell’Istruzione, dell’ Università e della Ricerca.
Availability of data and materials
All authors contributed to design the study, critically revised and approved the manuscript. PV, GL, TD’A, and ML collected and analyzed histological and immunohistochemical data. PF and MB collected and analyzed clinical data. LC and R Di P collected and analyzed morphometrical data. FOR and FBA performed statistical analyses. PV and FBA wrote the manuscript.
The authors declare no conflicts of interest.
Consent for publication
Informed consent was obtained from the patients.
Ethic approval and consent to participate
The study was conducted in accordance with the Declaration of Helsinki and has been approved by the author’s Institutional Review Board.